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replication factor-C

Function: - elongation of primed DNA templates by DNA polymerase delta & DNA polymerase epsilon requires the action of the accessory proteins PCNA & replication factor-C (activator 1) - complex containing RFC1 forms a complex with PCNA in the presence of ATP stimulating ATPase activity - complex containing RAD17 does not interact with PCNA Subunits: - heteropentamer of subunits: replication factor C subunit 1 (RFC1) replication factor C subunit 2 (RFC2) replication factor C subunit 3 (RFC3) replication factor C subunit 4 (RFC4) replication factor C subunit 5 (RFC5) - RFC2, RFC2, RFC4, & RFC5 may complex with either RFC1 or RAD17 [2] - RFC2, RFC2, RFC4, & RFC5 may complex with CTF18, CTF8, & DCC1 in the CTF18-RFC complex

Related

CTF18-replication factor C proliferating cell nuclear antigen (PCNA)

General

adenosine triphosphatase (ATPase) DNA-binding protein multisubunit protein nuclear protein

Figures/Diagrams

Model for nucleotide excision repair (v2)

Properties

COMPARTMENT: cell nucleus SUBUNITS: replication factor C subunit 1 MOTIF: Ser phosphorylation site {S69} Ser phosphorylation site {S71} Ser phosphorylation site {S73} Ser phosphorylation site {S108} Thr phosphorylation site {T110} Ser phosphorylation site {S156} Thr phosphorylation site {T161} Ser phosphorylation site {S173} Ser phosphorylation site {S190} BRCA1 C-terminal (BRCT) motif SITE: 402-492 ATP-binding site NAME: ATP-binding site SITE: 650-657 Zn finger C2HC-type SITE: 749-766 EFFECTOR-BOUND: Zn+2 nuclear translocation signal {1120-1124} replication factor C subunit 2 MOTIF: ATP-binding site NAME: ATP-binding site replication factor C subunit 3 MOTIF: Ser phosphorylation site {S125} replication factor C subunit 4 MOTIF: ATP-binding site NAME: ATP-binding site SITE: 78-85 replication factor C subunit 5 MOTIF: ATP-binding site NAME: ATP-binding site SITE: 60-67

References

  1. Linn S How many pols does it take to replicate nuclear DNA? Cell 66:185 1991 PMID: 1855251
  2. UniProt :accession P35250

Component-of

RAD17-RFC complex

Components

replication factor C subunit 1 (replication factor C p140, replication factor C large subunit, RF-C 140 kD subunit, activator 1 140 kD subunit, activator 1 large subunit, A1 140 kD subunit, DNA-binding protein PO-GA, RFC1, RFC140) replication factor C subunit 2 (replication factor C 40 kD subunit, RF-C 40 kD subunit, RFC40, activator 1 40 kD subunit, A1 40 kD subunit, RFC2) replication factor C subunit 3 (replication factor C p38, replication factor C 38 kD subunit, RFC38, activator 1 38 kD subunit, A1 38 kD subunit, RF-C 38 kD subunit, RFC3) replication factor C subunit 4 (replication factor C p37, replication factor C 37 kD subunit, RF-C 37 kD subunit, RFC37, activator 1 37 kD subunit, A1 37 kD subunit, RFC4) replication factor C subunit 5 (replication factor C p36, RF-C 36 kD subunit, RFC36, activator 1 36 kD subunit, A1 36 kD subunit, RFC5)