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replication factor-A (RFA/RPA)

Function: - single-stranded DNA-binding protein - role in DNA replication, homologous recombination & nucleotide excision repair [4] - increases processivity of DNA polymerase alpha & is required for efficient DNA polymerase delta activity - phosphorylation of RPA2 by p34cdc2 (CDK1) may activate RPA activity & DNA replication; hyperphosphorylation may inactivate RPA, halt replication & enhanc DNA repair. - DNA-binding activity may reside exclusively in RPA1 Structure: - heterotrimer a) RPA1 (70 kD) b) RPA2 (30/32 kD) c) RPA3 (14 kD) Pathology: - required for SV40 replication in vitro

General

DNA-binding protein multisubunit protein nuclear protein phosphoprotein

Figures/Diagrams

Model for nucleotide excision repair (v2)

Properties

COMPARTMENT: cell nucleus SUBUNITS: replication protein A 70 kD DNA-binding subunit MOTIF: Thr phosphorylation site {T180} DNA-binding motif SITE: 198-291 DNA-binding motif SITE: 305-402 MOTIF: Ser phosphorylation site {S384} Zn finger C4-type SITE: 481-503 EFFECTOR-BOUND: Zn+2 replication protein A 32 kD subunit MOTIF: serine-rich region {1-29} MOTIF: serine residue (SEVERAL) acetylation site SITE: N-TERMINUS EFFECTOR-BOUND: acetyl Thr phosphorylation site {T21} serine residue {23} Ser phosphorylation site {S29} Ser phosphorylation site {S33} lysine-rich region {37-45} MOTIF: lysine residue (SEVERAL) acidic region {95-123} MOTIF: acidic residue (SEVERAL) lysine-rich region {127-145} MOTIF: lysine residue (SEVERAL) TIPIN interaction {187-270} MOTIF: acidic region {247-270} MOTIF: acidic residue (SEVERAL) replication protein A 14 kD subunit

References

  1. Erdile et al J Biol Chem 265:3177 1990
  2. Linn S How many pols does it take to replicate nuclear DNA? Cell 66:185 1991 PMID: 1855251
  3. Gottlieb TM, Jackson SP. Protein kinases and DNA damage. Trends Biochem Sci. 1994 Nov;19(11):500-3. Review. PMID: 7855895
  4. He Z, Henricksen LA, Wold MS, Ingles CJ. RPA involvement in the damage-recognition and incision steps of nucleotide excision repair. Nature. 1995 Apr 6;374(6522):566-9. PMID: 7700386

Components

replication protein A 14 kD subunit; RP-A p14; replication factor A protein 3; RF-A protein 3 (RPA3, REPA3, RPA14) replication protein A 32 kD subunit; RP-A p32; RP-A p34; replication factor A protein 2; RF-A protein 2 (RPA2 REPA2 RPA32 RPA34) replication protein A 70 kD DNA-binding subunit; RP-A p70; replication factor A protein 1; RF-A protein 1; single-stranded DNA-binding protein (RPA1 REPA1 RPA70)