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rap guanine nucleotide exchange factor 3; cAMP-regulated guanine nucleotide exchange factor I; cAMP-GEFI; exchange factor directly activated by cAMP 1; exchange protein directly activated by cAMP 1; EPAC 1; rap1 guanine-nucleotide-exchange factor directly activated by cAMP (RAPGEF3, CGEF1, EPAC, EPAC1)
Function:
- guanine nucleotide exchange factor (GEF) for RAP1A & RAP2A small GTPases that is activated by binding cAMP
Structure:
- the DEP domain is involved in membrane localization independent from regulation by cAMP
- contains 1 cyclic nucleotide-binding domain
- contains 1 DEP domain
- contains 1 N-terminal Ras-GEF domain
- contains 1 Ras-GEF domain
Compartment:
- endomembrane system
Alternative splicing: named isoforms=2
Expression:
- widely expressed with highest levels in adult kidney, heart, thyroid & brain, & fetal kidney
Note:
- uncertain whether Met-1 or Met-43 is the initiator
General
guanine nucleotide exchange factor (GDP/GTP dissociation stimulator, guanine nucleotide-releasing factor/protein, GNEF, GNDS, GDS)
Properties
SIZE: MW = 99 kD
entity length = 881 aa
COMPARTMENT: cytoplasm
MOTIF: DEP domain {68-144}
cAMP-binding site
SITE: 203-323
guanine nucleotide exchange domain
NAME: guanine nucleotide exchange domain
SITE: 342-476
guanine nucleotide exchange domain
NAME: guanine nucleotide exchange domain
SITE: 620-840
Database Correlations
OMIM 606057
UniProt O95398
PFAM correlations
Entrez Gene 10411
Kegg hsa:10411
References
- de Rooij J et al
Epac is a Rap1 guanine-nucleotide-exchange factor directly
activated by cyclic AMP.
Nature 396:474-477, 1998
PMID: 9853756
- UniProt :accession O95398