Ran-binding protein 3 (RANBP3)

Function: - acts as a cofactor for XPO1/CRM1-mediated nuclear export, perhaps as export complex scaffolding protein - bound to XPO1/CRM1, stabilizes the XPO1/CRM1-cargo interaction - in the absence of Ran-bound GTP, prevents binding of XPO1/CRM1 to the nuclear pore complex - binds to CHC1/RCC1 & increases guanine nucleotide exchange activity of CHC1/RCC1 - recruits XPO1/CRM1 to CHC1/RCC1 (Ran-dependent) - negative regulator of TGF-beta signaling through interaction with the R-SMAD proteins, SMAD2 & SMAD3, & mediating their nuclear export - phosphorylated upon DNA damage, probably by ATM or ATR - interacts with CHC1 (Ran-stimulated) - interacts with XPO1 - interacts (via its C-terminal R domain) with SMAD2 (dephosphorylated form via its MH1 & MH2 domains) - the interaction results in the nuclear export of SMAD2 & termination of TGF-beta signaling - interacts (via its C-terminal R domain) with SMAD3 (dephosphorylated form via its MH1 domain) - the interaction results in the nuclear export of SMAD3 & termination of TGF-beta signaling Structure: contains 1 RanBD1 domain Compartment: cytoplasm, nucleus Alternative splicing: named isoforms=3 Expression: - widely expressed - highly expressed in testis & heart

General

cytoskeletal protein phosphoprotein

Properties

SIZE: entity length = 567 aa MW = 60 kD COMPARTMENT: cytoplasm cell nucleus MOTIF: acetylation site SITE: N-TERMINUS EFFECTOR-BOUND: acetyl proline-rich region SITE: 76-81 MOTIF: proline residue (SEVERAL) Ser phosphorylation site {S96} Ser phosphorylation site {S100} Ser phosphorylation site {S101} Ser phosphorylation site {S108} Tyr phosphorylation site {Y115} Thr phosphorylation site {T124} Ser phosphorylation site {S125} Ser phosphorylation site {S126} Ser phosphorylation site {S133} Ser phosphorylation site {S211} Thr phosphorylation site {T214} Ser phosphorylation site {S219} Ser phosphorylation site {S221} Ser phosphorylation site {S333} Ser phosphorylation site {S353} Ser phosphorylation site {S355} Ser phosphorylation site {S357} Ser phosphorylation site {S359} Ran binding domain type 1 {378-518} Ser phosphorylation site {S533} Ser phosphorylation site {S539}

Database Correlations

OMIM 603327 UniProt Q9H6Z4 Pfam PF00638 Entrez Gene 8498 Kegg hsa:8498

References

UniProt :accession Q9H6Z4