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raf1 proto-oncogene protein

Function: - both cytosolic & membrane bound raf1 are found in large (300-500 kD) complexes that include 2 chaperones, hsp90 & p50 - Raf1 kinase may be activated by GTP-bound p21ras & by protein kinase C [8] - Raf1 interacts with 14-3-3-beta & 14-3-3-zeta through its Zn finger [8] Compartment: cytoplasm, membrane Expression: ubiquitously expressed

Interactions

molecular events

Related

RAF1 gene mutation raf1 proto-oncogene ras signal transduction

General

raf proto-oncogene protein or mil proto-oncogene protein

Properties

SIZE: MW = 74 kD entity length = 648 aa COMPARTMENT: cytoplasm CELL: most cell types* MOTIF: domain MOTIF: Ser phosphorylation site {S43} cysteine residue (SEVERAL) binding site FOR-BINDING-OF: ras subfamily protein Zinc finger NAME: Zinc finger EFFECTOR-BOUND: Zn+2 HOMOLOGY: raf proto-oncogene protein* domain MOTIF: Ser phosphorylation site {S259} Thr phosphorylation site {T268} HOMOLOGY: raf proto-oncogene protein* kinase domain MOTIF: ATP-binding site NAME: ATP-binding site Ser phosphorylation site {S621} STATE: active state

Database Correlations

OMIM 164760 MORBIDMAP 164760 UniProt P04049

References

  1. Moodie SA, Willumsen BM, Weber MJ, Wolfman A. Complexes of Ras.GTP with Raf-1 and mitogen-activated protein kinase kinase. Science. 1993 Jun 11;260(5114):1658-61. PMID: 8503013
  2. Zhang XF, Settleman J, Kyriakis JM, Takeuchi-Suzuki E, Elledge SJ, Marshall MS, Bruder JT, Rapp UR, Avruch J. Normal and oncogenic p21ras proteins bind to the amino- terminal regulatory domain of c-Raf-1. Nature. 1993 Jul 22;364(6435):308-13. PMID: 8332187
  3. Morrison DK, Heidecker G, Rapp UR, Copeland TD. Identification of the major phosphorylation sites of the Raf-1 kinase. J Biol Chem. 1993 Aug 15;268(23):17309-16. PMID: 8349614
  4. Bonner et al Nucleic Acids Res 14:1009 1993
  5. Vojtek AB, Hollenberg SM, Cooper JA. Mammalian Ras interacts directly with the serine/threonine kinase Raf. Cell. 1993 Jul 16;74(1):205-14. PMID: 8334704
  6. Molecular Cell Biology (2nd ed) Darnell J; Lodish H & Baltimore D (eds), Scientific American Books, WH Freeman, NY 1990, pg 986
  7. Hall A. A biochemical function for ras--at last. Science. 1994 Jun 3;264(5164):1413-4. PMID: 8197454
  8. Aitken A. 14-3-3 proteins on the MAP. Trends Biochem Sci. 1995 Mar;20(3):95-7. PMID: 7709434

Component-of

molecular complex