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P-selectin glycoprotein ligand 1; PSGL-1; selectin P ligand; CD162 (SELPLG)

Function: 1) SLe(x)-type glycan 2) high affinity Ca+2-dependent interactions with selectins a) E-selection b) P-selectin c) L-selectin 3) via 2, mediates rapid rolling of leukocytes over vascular surfaces during the initial steps in inflammation 4) PSGL1 is critical for the initial leukocyte capture 5) interacts with SNX20 Structure: - homodimer; disulfide-linked - complex, core-2, sialylated & fucosylated O-linked oligosaccharides, at least some of which appear to contain poly-N-acetyllactosamine with varying degrees of substitution - disialylated or neutral forms of core-2 tetrasaccharide: galbeta1-->4GlcNAcbeta1-->6(galbeta1-->3)galNAcOH - GlcN:galN ratio is approximately 2:1 - man:fuc ratio 3:5. - contains about 14% fucose with alpha-1,3 linkage present in two forms: a) disialylated, monofucosylated glycan b) monosialylated, trifucosylated glycan with polylactosamine backbone - fucosylated forms carry the Lewis antigen & are important for interaction with selectins & for functioning in leukocyte rolling - modification containing sialyl Lewis X glycan is on Thr-57 - no sulfated O-glycans - interaction with P-selectin, E-selectin & L-selectins, through their lectin/EGF domains, is required for promoting recruitment & rolling of leukocytes - these interactions require sialyl Lewis X glycan modification but there is a differing dependence for Tyr sulfations - high affinity P-selectin binding has a preferred requirement for the isomer sulfated on both Tyr-48 & Tyr-51 (dimerization appears not to be required) - L-selectin binding requires predominantly sulfation on Tyr-51 with sulfation on Tyr-48 playing only a minor role - E-selectin/SELE binds with much lower affinity & requires SLe(x) epitope, but apparantly not Tyr sulfation Compartment: - membrane; single-pass type 1 membrane protein Expression: - expressed on neutrophils, monocytes & most lymphocytes

General

cluster-of-differentiation antigen; cluster designation antigen; CD antigen glycoprotein phosphoprotein

Properties

SIZE: entity length = 412 aa MW = 43 kD COMPARTMENT: cellular membrane MOTIF: signal sequence {1-17} Thr glycosylation site {T57} N-glycosylation site {N65} N-glycosylation site {N111} 12 X 10 AA tandem repeats {122-261} MOTIF: consensus repeat {122-131} consensus repeat {132-141} consensus repeat {142-151} consensus repeat {162-171} consensus repeat {182-191} consensus repeat {192-201} consensus repeat {202-211} consensus repeat {212-221} consensus repeat {222-231} consensus repeat {232-241} consensus repeat {242-251} consensus repeat {252-261} N-glycosylation site {N302} cysteine residue {C320} MODIFICATION: cysteine residue {C-INTERCHAIN} transmembrane domain {321-341} Ser phosphorylation site {S389} Thr phosphorylation site {T393}

Database Correlations

OMIM 600738 UniProt Q14242 Entrez Gene 6404 Kegg hsa:6404

References

  1. UniProt :accession Q14242
  2. Wikipedia: P-selectin glycoprotein ligand 1 http://en.wikipedia.org/wiki/P-selectin_glycoprotein_ligand-1
  3. SeattleSNPs http://pga.gs.washington.edu/data/selplg/