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transcription factor PU.1; 31 kD-transforming protein (SPI1)

Function: - binds to the PU-box, a purine-rich DNA sequence (5'-GAGGAA-3') that can act as a lymphoid-specific enhancer - transcriptional activator that may be specifically involved in the differentiation or activation of macrophages or B-cells - also binds RNA & may modulate pre-mRNA splicing - binds DNA as a monomer - interacts with RUNX1 & SPIB - interacts with CEBPD & NONO (putative) Structure: - belongs to the ETS family - contains 1 ETS DNA-binding domain Compartment: nucleus Expression: - normal expression of SPI1 is restricted to B-cells & macrophages Pathology: - highly expressed in both FV-P & FV-A-induced erythroleukemia cell lines that have undergone rearrangements of the spi-1 gene due to the insertion of SFFV

Interactions

molecular events

Related

spi1 proto-oncogene, ets-related gene or PU-1

General

DNA-binding protein phosphoprotein transcription factor (TF)

Properties

SIZE: MW = 30 kD COMPARTMENT: cell nucleus CELL: B lymphocyte macrophage MOTIF: ETS domain NAME: ETS domain Ser phosphorylation site {S148}

Database Correlations

OMIM 165170 UniProt P17947 Pfam PF00178

References

  1. Bishop JM. Molecular themes in oncogenesis. Cell. 1991 Jan 25;64(2):235-48. Review. PMID: 1988146
  2. Macleod K, Leprince D, Stehelin D. The ets gene family. Trends Biochem Sci. 1992 Jul;17(7):251-6. Review. PMID: 1502727
  3. Pongubala JM, Van Beveren C, Nagulapalli S, Klemsz MJ, McKercher SR, Maki RA, Atchison ML. Effect of PU.1 phosphorylation on interaction with NF-EM5 and transcriptional activation. Science. 1993 Mar 12;259(5101):1622-5. PMID: 8456286
  4. Atlas of genetics & cytogenetics in oncology & haematology http://atlasgeneticsoncology.org/genes/SPI1ID269.html

Component-of

molecular complex