protein synthesis elongation

Biochemistry: * see figure 1 - protein synthesis 7 - substrate: elongation factor 1 + GTP - product: eF1-alpha (GTP) + eF1-beta - protein synthesis 8 - substrate: eF1-alpha (GTP) + tRNA :amino acyl amino acid residue - product: molecular complex - subunits: eF1-alpha (GTP) + tRNA :amino acyl amino acid residue - protein synthesis 9 - substrate: molecular complex - subunits: eF1-alpha (GTP) + tRNA :amino acyl amino acid residue + 80S-initiation complex - product: 80S-initiation complex :a-site transfer RNA :amino acyl amino acid residue :p-site transfer RNA :amino acyl met + eF1-alpha (GDP) + H2PO4- * see figure 2 - protein synthesis 10 - substrate: eF1-alpha (GDP) + eF1-beta - product: elongation factor 1 + GDP - protein synthesis 11 - enzyme: peptidyl transferase - substrate: 80S-initiation complex :a-site transfer RNA :amino acyl amino acid residue :p-site transfer RNA :amino acyl met - product: 80S-initiation complex :a-site transfer RNA :amino acyl peptide amino acid residue - met :p-site transfer RNA :amino acyl unbound * see figure 3 - protein synthesis 12 - substrate: 80S-initiation complex :a-site transfer RNA :amino acyl peptide amino acid residue - met :p-site transfer RNA :amino acyl unbound + elongation factor 2 - product: 80S-elongation complex :a-site unbound :p-site transfer RNA :amino acyl peptide + elongation factor 2 (GDP) + H2PO4- - protein synthesis 13 - enzyme: GDP-GTP dissociation stimulator - substrate: elongation factor 2 (GDP, GTP) - product: elongation factor 2 + GDP * see figure 4 & figure 7 - protein synthesis 14 - substrate: molecular complex - subunits: eF1-alpha (GTP) + tRNA :amino acyl amino acid residue + 80S-elongation complex :a-site unbound :p-site transfer RNA :amino acyl peptide - product: 80S-elongation complex :a-site transfer RNA :amino acyl amino acid residue :p-site transfer RNA :amino acyl peptide + eF1-alpha (GDP) + H2PO4- * see figure 5 - protein synthesis 15 - enzyme: peptidyl transferase - substrate: 80S-elongation complex :a-site transfer RNA :amino acyl amino acid residue :p-site transfer RNA :amino acyl peptide - product: 80S-elongation complex :a-site transfer RNA :amino acyl peptide :p-site transfer RNA :amino acyl unbound * see figure 6 - protein synthesis 16 - substrate: 80S-elongation complex :a-site transfer RNA :amino acyl peptide :p-site transfer RNA :amino acyl unbound + elongation factor 2 - product: 80S-elongation complex :a-site unbound :p-site transfer RNA :amino acyl peptide + elongation factor 2 (GDP) + H2PO4- * figures 4 & 7 are the same representing repetition of polymerization

Properties

EVENTS: chemical reaction SUBSTRATE: elongation factor 1 COMPARTMENT: cytoplasm CELL: most cell types* ORGANISM: eukaryote* guanosine triphosphate PRODUCT: elongation factor 1-alpha COMPARTMENT: cytoplasm CELL: most cell types* ORGANISM: eukaryote* MOTIF: P-loop FOR-BINDING-OF: guanosine triphosphate guanine nucleotide-binding site EFFECTOR-BOUND: guanosine triphosphate elongation factor 1-beta COMPARTMENT: cytoplasm CELL: most cell types* ORGANISM: eukaryote* MOTIF: guanine nucleotide exchange domain NAME: guanine nucleotide exchange domain TAG: PROTEIN-SYNTHESIS-7 binding SUBSTRATE: elongation factor 1-alpha COMPARTMENT: cytoplasm CELL: most cell types* ORGANISM: eukaryote* MOTIF: P-loop FOR-BINDING-OF: guanosine triphosphate guanine nucleotide-binding site EFFECTOR-BOUND: guanosine triphosphate transfer RNA COMPARTMENT: cytoplasm MOTIF: anticodon loop AMINO-ACYL: amino acid residue PRODUCT: molecular complex COMPARTMENT: cytoplasm TAG: PROTEIN-SYNTHESIS-8 chemical reaction SUBSTRATE: molecular complex COMPARTMENT: cytoplasm 80S initiation complex COMPARTMENT: cytoplasm MOTIF: active site allosteric site P-SITE: transfer RNA MOTIF: anticodon loop AMINO-ACYL: methionine residue PRODUCT: 80S initiation complex COMPARTMENT: cytoplasm MOTIF: active site allosteric site P-SITE: transfer RNA MOTIF: anticodon loop AMINO-ACYL: methionine residue A-SITE: transfer RNA MOTIF: anticodon loop AMINO-ACYL: amino acid residue elongation factor 1-alpha COMPARTMENT: cytoplasm CELL: most cell types* ORGANISM: eukaryote* MOTIF: P-loop FOR-BINDING-OF: guanosine triphosphate guanine nucleotide-binding site EFFECTOR-BOUND: guanosine diphosphate H2PO4- TAG: PROTEIN-SYNTHESIS-9 binding SUBSTRATE: elongation factor 1-alpha COMPARTMENT: cytoplasm CELL: most cell types* ORGANISM: eukaryote* MOTIF: P-loop FOR-BINDING-OF: guanosine triphosphate guanine nucleotide-binding site EFFECTOR-BOUND: guanosine diphosphate elongation factor 1-beta COMPARTMENT: cytoplasm CELL: most cell types* ORGANISM: eukaryote* MOTIF: guanine nucleotide exchange domain NAME: guanine nucleotide exchange domain PRODUCT: elongation factor 1 COMPARTMENT: cytoplasm CELL: most cell types* ORGANISM: eukaryote* guanosine diphosphate TAG: PROTEIN-SYNTHESIS-10 enzymatic reaction ENZYME: peptidyl transferase COMPARTMENT: cytoplasm CELL: most cell types* ORGANISM: eukaryote* MOTIF: active site SUBSTRATE: 80S initiation complex COMPARTMENT: cytoplasm MOTIF: active site allosteric site P-SITE: transfer RNA MOTIF: anticodon loop AMINO-ACYL: methionine residue A-SITE: transfer RNA MOTIF: anticodon loop AMINO-ACYL: amino acid residue PRODUCT: 80S initiation complex COMPARTMENT: cytoplasm MOTIF: active site allosteric site P-SITE: transfer RNA MOTIF: anticodon loop A-SITE: transfer RNA MOTIF: anticodon loop AMINO-ACYL: peptide PEPTIDE-SEQUENCE: amino acid residue methionine residue TAG: PROTEIN-SYNTHESIS-11 chemical reaction SUBSTRATE: 80S initiation complex COMPARTMENT: cytoplasm MOTIF: active site allosteric site P-SITE: transfer RNA MOTIF: anticodon loop A-SITE: transfer RNA MOTIF: anticodon loop AMINO-ACYL: peptide PEPTIDE-SEQUENCE: amino acid residue methionine residue elongation factor 2 COMPARTMENT: cytoplasm CELL: most cell types* ORGANISM: eukaryote* MOTIF: S/T phosphorylation site binding site FOR-BINDING-OF: G-alpha P-loop EFFECTOR-BOUND: guanosine triphosphate PRODUCT: 80S elongation complex COMPARTMENT: cytoplasm MOTIF: active site allosteric site P-SITE: transfer RNA MOTIF: anticodon loop AMINO-ACYL: peptide elongation factor 2 COMPARTMENT: cytoplasm CELL: most cell types* ORGANISM: eukaryote* MOTIF: S/T phosphorylation site binding site FOR-BINDING-OF: G-alpha P-loop EFFECTOR-BOUND: guanosine triphosphate guanine nucleotide-binding site EFFECTOR-BOUND: guanosine diphosphate H2PO4- TAG: PROTEIN-SYNTHESIS-12 enzymatic reaction ENZYME: guanine nucleotide exchange factor MOTIF: guanine nucleotide exchange domain NAME: guanine nucleotide exchange domain SUBSTRATE: elongation factor 2 COMPARTMENT: cytoplasm CELL: most cell types* ORGANISM: eukaryote* MOTIF: S/T phosphorylation site binding site FOR-BINDING-OF: G-alpha P-loop EFFECTOR-BOUND: guanosine triphosphate guanine nucleotide-binding site EFFECTOR-BOUND: guanosine diphosphate guanosine triphosphate PRODUCT: elongation factor 2 COMPARTMENT: cytoplasm CELL: most cell types* ORGANISM: eukaryote* MOTIF: S/T phosphorylation site binding site FOR-BINDING-OF: G-alpha P-loop EFFECTOR-BOUND: guanosine triphosphate guanosine diphosphate TAG: PROTEIN-SYNTHESIS-13 chemical reaction SUBSTRATE: molecular complex 80S elongation complex COMPARTMENT: cytoplasm MOTIF: active site allosteric site P-SITE: transfer RNA MOTIF: anticodon loop AMINO-ACYL: peptide PRODUCT: 80S elongation complex COMPARTMENT: cytoplasm MOTIF: active site allosteric site P-SITE: transfer RNA MOTIF: anticodon loop AMINO-ACYL: peptide A-SITE: transfer RNA MOTIF: anticodon loop AMINO-ACYL: amino acid residue elongation factor 1-alpha COMPARTMENT: cytoplasm CELL: most cell types* ORGANISM: eukaryote* MOTIF: P-loop FOR-BINDING-OF: guanosine triphosphate guanine nucleotide-binding site EFFECTOR-BOUND: guanosine diphosphate H2PO4- TAG: PROTEIN-SYNTHESIS-14 enzymatic reaction ENZYME: peptidyl transferase COMPARTMENT: cytoplasm CELL: most cell types* ORGANISM: eukaryote* MOTIF: active site SUBSTRATE: 80S elongation complex COMPARTMENT: cytoplasm MOTIF: active site allosteric site P-SITE: transfer RNA MOTIF: anticodon loop AMINO-ACYL: peptide A-SITE: transfer RNA MOTIF: anticodon loop AMINO-ACYL: amino acid residue PRODUCT: 80S elongation complex COMPARTMENT: cytoplasm MOTIF: active site allosteric site P-SITE: transfer RNA MOTIF: anticodon loop A-SITE: transfer RNA MOTIF: anticodon loop AMINO-ACYL: peptide TAG: PROTEIN-SYNTHESIS-15 chemical reaction SUBSTRATE: 80S elongation complex COMPARTMENT: cytoplasm MOTIF: active site allosteric site P-SITE: transfer RNA MOTIF: anticodon loop A-SITE: transfer RNA MOTIF: anticodon loop AMINO-ACYL: peptide elongation factor 2 COMPARTMENT: cytoplasm CELL: most cell types* ORGANISM: eukaryote* MOTIF: S/T phosphorylation site binding site FOR-BINDING-OF: G-alpha P-loop EFFECTOR-BOUND: guanosine triphosphate PRODUCT: 80S elongation complex COMPARTMENT: cytoplasm MOTIF: active site allosteric site P-SITE: transfer RNA MOTIF: anticodon loop AMINO-ACYL: peptide elongation factor 2 COMPARTMENT: cytoplasm CELL: most cell types* ORGANISM: eukaryote* MOTIF: S/T phosphorylation site binding site FOR-BINDING-OF: G-alpha P-loop EFFECTOR-BOUND: guanosine triphosphate guanine nucleotide-binding site EFFECTOR-BOUND: guanosine diphosphate H2PO4- TAG: PROTEIN-SYNTHESIS-16