protein kinase G type-1; cGMP-dependent protein kinase type-1; cGMP-dependent protein kinase alpha (PRKG1, PRKGR1A, PRKG1B, PRKGR1B)

Function: - serine/threonine protein kinase - binding of cGMP results in enzyme activation - substrate specificity similar but not identical to CAK - part of cGMP kinase signaling complex at least composed of ACTA2/alpha-actin, CNN1/calponin H1, PLN/phospholamban, PRKG1 & ITPR1 (putative) - interacts with MRVI1 Structure: - antiparallel homodimer - belongs to the protein kinase superfamily, AGC Ser/Thr protein kinase family, cGMP subfamily - contains 1 AGC-kinase C-terminal domain - contains 2 cyclic nucleotide-binding domains - contains 1 protein kinase domain Alternative splicing: named isoforms=2 Genetics: - cGMP protein kinase-1 alpha & beta subunits produced from same transcript by alternative splicing.

General

allosteric enzyme myristoylated protein oligomerizing protein protein kinase G (PKG)

Properties

SIZE: entity length = 671 aa MW = 76 kD COMPARTMENT: cellular membrane STATE: active state MOTIF: Dimerization {2-102} MOTIF: cysteine residue {C43} MODIFICATION: cysteine residue {C-INTERCHAIN} Thr phosphorylation site {T59} cGMP-binding site SITE: 103-220 cGMP-binding site SITE: 221-341 kinase domain SITE: 360-619 MOTIF: ATP-binding site NAME: ATP-binding site SITE: 366-374 ATP-binding site NAME: ATP-binding site SITE: 390-390 aspartate residue {D484} AGC-kinase C-terminal {620-671} MISC-INFO: DISTRIBUTION :REGIONAL RESTRICTED :SUBCELLULAR CYTOSOL SUBSTRATE-SPECIFICITY :RANGE NARROW RECOGNITION-MOTIF :SEQUENCE X[S*]RX :REF " - Kemp BE, Pearson RB. Protein kinase recognition sequence motifs. Trends Biochem Sci. 1990 Sep;15(9):342-6. Review. PMID: 2238044"

Database Correlations

OMIM 176894 UniProt Q13976 PFAM correlations Kegg hsa:5592 ENZYME 2.7.11.12