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E3 SUMO-protein ligase PIAS2; androgen receptor-interacting protein 3; ARIP3; DAB2-interacting protein; DIP; Msx-interacting zinc finger protein; Miz1; PIAS-NY protein; protein inhibitor of activated STAT x; protein inhibitor of activated STAT2 (PIAS2, PIASX)
Function:
1) SUMO-ligase E3
2) stabilizes interaction between UBE2I & substrate
3) SUMO-tethering factor
4) transcriptional coregulator in
a) STAT pathway
b) p53 pathway
c) steroid hormone signaling pathway
5) gene silencing
6) binds to sumoylated ELK1 & enhances its transcriptional activity
7) sumoylation appears to enhance the PIASX interactions
8) binds SUMO1 & UBE2I
9) interacts with JUN, MDM2, PARK7, TP53, TP73 isoform alpha, STAT4 following IL12 & IFN-alpha stimulation of T-cells, GTF2I, GTF2IRD1, DAB2, MSX2, ESR1, ESR2, NR3C1, PGR, AR, NCOA2
10) binds to CDKN1A promoter
Compartment: nucleus, nuclear speckles
Alternative splicing: named isoforms=3
Expression:
- expressed mainly in testis
- up-regulated transiently during myeloid differentiation in cells lines induced by phorbol ester or retinoic acid
General
nuclear protein
ring finger protein
SUMO ligase E3 (sentrin ligase E3)
Properties
SIZE: entity length = 650 aa
COMPARTMENT: cell nucleus
MOTIF: SAP {11-45}
RING-finger {331-408}
EFFECTOR-BOUND: Zn+2
FOR-BINDING-OF: DNA motif
active site
binding site
SITE: 472-482
FOR-BINDING-OF: small ubiquitin-related modifier 1
nuclear translocation signal {484-492}
serine-rich region {571-612}
MOTIF: serine residue (SEVERAL)
serine-rich region {596-602}
MOTIF: serine residue (SEVERAL)
Database Correlations
OMIM 603567
UniProt O75928
Pfam PF02891
Entrez Gene 9063
Kegg hsa:9063
References
- OMIM :accession 603567
- UniProt :accession O75928