multivesicular body subunit 12A; CIN85/CD2AP family-binding protein; ESCRT-I complex subunit MVB12A; protein FAM125A (MVB12A, CFBP, FAM125A)

Function: - component of the ESCRT-1 complex, a regulator of vesicular trafficking process - required for sorting of endocytic ubiquitinated cargos into multivesicular bodies - may be involved in the ligand-mediated internalization & down-regulation of EGF receptor - phosphorylated on Tyr-204 upon EGF stimulation - phosphorylation is required for interaction with CD2AP & CIN85/SH3KBP1 - interacts with CD2AP & CIN85/SH3KBP1 - interacts with CD2AP (via one of the SH3 domains) - within the ESCRT-1 complex - interacts with VPS28 - interacts with TSG101, VPS37B, VPS37C, & VPS37D - association appears to be mediated by the TSG101-VPS37 binary subcomplex - interacts with CEP55 - does not interact with CD2AP Structure: - belongs to the MVB12 family - contains 1 MABP domain - contains 1 UMA domain Compartment: - cytoplasm, nucleus - endosome, late endosome membrane - cytoskeleton, microtubule organizing center, centrosome - colocalizes with F-actin - some fraction may be nuclear Alternative splicing: named isoforms=3 Expression: - ubiquitously expressed - not expressed in skeletal muscle

General

cytoskeletal protein phosphoprotein

Properties

SIZE: entity length = 273 aa MW = 29 kD COMPARTMENT: cytoplasm cell nucleus MOTIF: MABP {9-151} MOTIF: Thr phosphorylation site {T130} SH3-binding site NAME: SH3-binding site SITE: 155-160 Ser phosphorylation site {S163} Ser phosphorylation site {S170} TSG101/VPS37B /VPS28 interaction {192-273} MOTIF: Ser phosphorylation site {S202} Tyr phosphorylation site {Y204} Ser phosphorylation site {S207} UMA {215-265} Ser phosphorylation site {S232}

Database Correlations

UniProt Q96EY5 Pfam PF10240 Entrez Gene 93343 Kegg hsa:93343

References

UniProt :accession Q96EY5