protein arginine N-methyltransferase 1; histone-arginine N-methyltransferase PRMT1; interferon receptor 1-bound protein 4 (PRMT1, HMT2, HRMT1L2, IR1B4)

Function: - protein arginine N-methyltransferase - methylates (mono & asymmetric dimethylation) the guanidino nitrogens of Arg present in proteins including ESR1, histone H2, H3 & H4, PIAS1, HNRNPA1, HNRNPD, NFATC2IP, SUPT5H, TAF15 & EWS - main enzyme that mediates monomethylation & asymmetric dimethylation of histone H4 Arg-4 (H4R3me1 & H4R3me2a, respectively), a specific tag for epigenetic transcriptional activation - together with dimethylated PIAS1, represses STAT1 transcriptional activity, in the late phase of interferon gamma (IFN-gamma) signaling - may play role in regulation of TAF15 transcriptional activity - may act as an activator of estrogen receptor-mediated transactivation - may play a role in neurite outgrowth - may negatively regulate megakaryocytic differentiation, by modulating p38 MAPK pathway - homodimer & heterodimer with PRMT8; the dimer can then associate to form a homohexamer - interacts with ILF3, BTG1, BTG2, SUPT5H & interferon-alpha/beta receptor 1 - interacts with NFATC2IP (putative) - activity regulated by BTG1, BTG2 & ILF3 S-adenosyl-L-methionine + arginine-[histone] = S-adenosyl-L-homocysteine + N(omega)-methyl-arginine-[histone] Kinetic parameters: - KM=1 uM for AdoMet - KM=4.2 uM for H4 - Vmax=1.2 nmol/min/mg enzyme toward AdoMet - Vmax=1.24 nmol/min/mg enzyme toward H4 Structure: - belongs to the protein arginine N-methyltransferase family Compartment: nucleus, cytoplasm (putative) Alternative splicing: named isoforms=3 Expression: widely expressed

General

nuclear protein phosphoprotein protein arginine N-methyltransferase (PRMT) receptor

Properties

SIZE: entity length = 361 aa MW = 42 kD COMPARTMENT: cytoplasm cell nucleus MOTIF: binding site SITE: 53-53 FOR-BINDING-OF: S-adenosylmethionine binding site SITE: 62-62 FOR-BINDING-OF: S-adenosylmethionine binding site SITE: 86-86 FOR-BINDING-OF: S-adenosylmethionine binding site SITE: 108-108 FOR-BINDING-OF: S-adenosylmethionine binding site SITE: 137-137 FOR-BINDING-OF: S-adenosylmethionine Tyr phosphorylation site {Y299}

Database Correlations

OMIM 602950 UniProt Q99873 Pfam PF06325 Entrez Gene 3276 ENZYME correlations

References

UniProt :accession Q99873