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polypeptide N-acetylgalactosaminyltransferase-like protein 3 (protein-UDP acetylgalactosaminyltransferase-like protein 3, UDP-GalNAc:polypeptide N-acetylgalactosaminyltransferase-like protein 3, polypeptide GalNAc transferase-like protein 3, GalNAc-T-like protein 3, pp-GaNTase-like protein 3, Williams Beuren syndrome chromosome region 17 protein, WBSCR17, GALNTL3)
Function:
- glycosylation
- may catalyze the initial reaction in O-linked oligosaccharide biosynthesis, transfer of an N-acetyl-D-galactosamine residue Ser or Thr on glycoprotein
UDP-N-acetyl-D-galactosamine + polypeptide UDP + N-acetyl-D-galactosaminyl-polypeptide
Cofactor: Mn+2, Ca+2
Structure:
- 2 conserved domains in the glycosyltransferase region
a) N-terminal domain (domain A, GT1 motif)
- Mn+2 coordination & substrate binding
b) C-terminal domain (domain B, Gal/GalNAc-T motif)
- catalytic reaction & UDP-Gal binding
- ricin B-type lectin domain binds to GalNAc & contributes to glycopeptide specificity
- belongs to the glycosyltransferase 2 family, GalNAc-T subfamily
Compartment: Golgi membrane
Expression:
- expressed in brain, heart
- weakly expressed in kidney, liver, lung, spleen
General
glycoprotein
glycosyltransferase
Properties
SIZE: MW = 68 kD
entity length = 598 aa
COMPARTMENT: golgi
MOTIF: transmembrane domain {7-27}
N-glycosylation site {N50}
active site
SITE: 151-262
active site
SITE: 319-381
N-glycosylation site {N461}
Ricin B-type lectin {465-594}
MOTIF: cysteine residue {C478}
MODIFICATION: cysteine residue {C494}
N-glycosylation site {N486}
cysteine residue {C494}
MODIFICATION: cysteine residue {C478}
cysteine residue {C526}
MODIFICATION: cysteine residue {C541}
cysteine residue {C541}
MODIFICATION: cysteine residue {C526}
cysteine residue {C568}
MODIFICATION: cysteine residue {C586}
cysteine residue {C586}
MODIFICATION: cysteine residue {C568}
Database Correlations
UniProt Q6IS24
PFAM correlations
ENZYME 2.4.1.41
References
- UniProt :accession Q6IS24
- GlycoGene database
http://ggdb.muse.aist.go.jp/GGDB/index.jsp