polycomb protein EED; hEED; WD protein associating with integrin cytoplasmic tails 1; WAIT-1 (EED)

Function: - polycomb group (PcG) protein - component of the PRC2/EED- EZH2 complex, which methylates Lys-9 & Lys-27 of histone H3, leading to transcriptional repression of the affected target gene - the PRC2/EED-EZH2 complex may also serve as a recruiting platform for DNA methyltransferases, thereby linking two epigenetic repression systems - genes repressed by the include HOXC8, HOXA9, MYT1 & CDKN2A - interacts with MLL (putative) - component of the PRC2/EED-EZH2 complex - interacts with HDAC, HDAC2, histone H1 & YY1 - may interact with ITGA4, ITGAE & ITGB7 - may interact with the MA protein of HIV-1 Structure: - belongs to the WD repeat ESC family - contains 7 WD repeats Compartment: - nucleus - transiently colocalizes withXIST at inactive X chromosomes Alternative splicing: - named isoforms=3 - additional isoforms may be produced by alternative initiation, both from non-canonical start codons upstream of the initiator Met displayed & from other canonical start codons downstream of that displayed; the precise sites of translation initiation have not been unambiguously identified Expression: - expressed in brain, colon, heart, kidney, liver, lung, muscle, ovary, peripheral blood leukocytes, pancreas, placenta, prostate, spleen, small intestine, testis, thymus & uterus. - expression peaks at the G1/S phase boundary - expression is induced by E2F1, E2F2 & E2F3 Pathology: - appears to be overexpressed in breast & colon cancer

General

nuclear protein WD repeat protein family

Properties

SIZE: entity length = 441 aa MW = 50 kD COMPARTMENT: cell nucleus MOTIF: EZH2 interaction {81-441} MOTIF: WD repeat {91-134} WD repeat {142-185} HIV1-MA interaction {149-303} WD repeat {188-228} WD repeat {234-275} HIV1-MA interaction {301-441} WD repeat {304-341} WD repeat {359-399} WD repeat {408-441}

Database Correlations

UniProt O75530 Pfam PF00400 Kegg hsa:8726

References

UniProt :accession O75530