Polycomb group multiprotein PRC1 complex (PcG PRC1 complex, hPRC-H, PcG repressive complex 1)

Function: 1) maintenance of transcriptionally repressive state of genes, including Hox genes, throughout development 2) acts via chromatin remodeling & modification of histones, rendering chromatin heritably changed in its expressibility 3) mediates monoubiquitination of histone H2A Lys-119, rendering chromatin heritably changed in its expressibility 4) RNF2 acts as the main E3 ubiquitin ligase on histone H2A 5) RING1 & PCGF4 act as modulators of RNF2 activity Structure: - components: - PCGF2/RNF110, PCGF4/BMI1, CBX2/M33, CBX4/PC2, CBX8/PC3, PHC1, PHC2, PHC3, SCMH1, RING1 & RNF2/RING2

General

molecular complex nuclear protein

Properties

COMPARTMENT: cell nucleus SUBUNITS: E3 ubiquitin-protein ligase RING2 MOTIF: HIP2 interaction {1-179} MOTIF: RING-finger {51-91} EFFECTOR-BOUND: Zn+2 FOR-BINDING-OF: DNA motif Ser phosphorylation site {S168} Ser phosphorylation site {S203} E3 ubiquitin-protein ligase RING1 COMPARTMENT: cell nucleus MOTIF: silencer element SITE: 30-234 MOTIF: Ser phosphorylation site {S38} RING-finger {48-88} EFFECTOR-BOUND: Zn+2 FOR-BINDING-OF: DNA motif Ser phosphorylation site {S187} Ser phosphorylation site {S188} Ser phosphorylation site {S190} nuclear translocation signal {201-204} glycine-rich region {205-260} CBX2 interaction {230-406} MOTIF: glycine-rich region {314-377} polycomb complex protein BMI-1 COMPARTMENT: cytoplasm cell nucleus MOTIF: RING-finger {18-57} EFFECTOR-BOUND: Zn+2 FOR-BINDING-OF: DNA motif nuclear translocation signal {81-95} E4F1 interaction {164-228} serine-rich region {251-326} MOTIF: serine residue (SEVERAL) Ser phosphorylation site {S251} Ser phosphorylation site {S253} Ser phosphorylation site {S255} polycomb group RING finger protein 2 COMPARTMENT: cell nucleus MOTIF: RING-finger {18-57} EFFECTOR-BOUND: Zn+2 FOR-BINDING-OF: DNA motif nuclear translocation signal {81-95} serine-rich region {242-344} MOTIF: serine residue (SEVERAL) chromobox protein homolog 2 COMPARTMENT: cell nucleus MOTIF: chromodomain NAME: chromodomain SITE: 12-70 FOR-BINDING-OF: heterochromatin A+T hook SITE: 75-87 serine-rich region {87-120} MOTIF: serine residue (SEVERAL) nuclear translocation signal {163-168} serine-rich region {464-469} MOTIF: serine residue (SEVERAL) E3 SUMO-protein ligase CBX4 COMPARTMENT: cell nucleus MOTIF: chromodomain NAME: chromodomain SITE: 11-69 FOR-BINDING-OF: heterochromatin Ser phosphorylation site {S90} histidine-rich region {378-400} MOTIF: histidine residue (SEVERAL) Ser phosphorylation site {S415} Thr phosphorylation site {T497} alanine-rich region {499-510} MOTIF: alanine residue (SEVERAL) chromobox protein homolog 8 COMPARTMENT: cell nucleus MOTIF: acetylation site SITE: N-TERMINUS EFFECTOR-BOUND: acetyl Ser phosphorylation site {S4} chromodomain NAME: chromodomain SITE: 11-69 FOR-BINDING-OF: heterochromatin Ser phosphorylation site {S110} Ser phosphorylation site {S130} Ser phosphorylation site {S256} Ser phosphorylation site {S265} Ser phosphorylation site {S311} Ser phosphorylation site {S332} Ser phosphorylation site {S352} polyhomeotic-like protein 1 COMPARTMENT: cell nucleus MOTIF: glutamine-rich region {425-441} MOTIF: glutamine residue (SEVERAL) Zinc finger NAME: Zinc finger SITE: 791-825 EFFECTOR-BOUND: Zn+2 sterile alpha motif NAME: sterile alpha motif SITE: 940-1004 polyhomeotic-like protein 2 COMPARTMENT: cell nucleus MOTIF: serine-rich region {11-37} MOTIF: serine residue (SEVERAL) glutamine-rich region {55-111} MOTIF: glutamine residue (SEVERAL) glutamine-rich region {329-377} MOTIF: glutamine residue (SEVERAL) HD1 {558-587} Zinc finger NAME: Zinc finger SITE: 633-667 EFFECTOR-BOUND: Zn+2 Ser phosphorylation site {S745} sterile alpha motif NAME: sterile alpha motif SITE: 794-858 polyhomeotic-like protein 3 COMPARTMENT: cell nucleus MOTIF: threonine-rich region {19-26} MOTIF: threonine residue (SEVERAL) serine-rich region {89-287} MOTIF: serine residue (SEVERAL) Ser phosphorylation site {S229} Ser phosphorylation site {S263} glutamine-rich region {324-539} MOTIF: glutamine residue (SEVERAL) proline-rich region SITE: 346-670 MOTIF: proline residue (SEVERAL) Thr phosphorylation site {T609} Ser phosphorylation site {S616} HD1 {691-720} Zinc finger NAME: Zinc finger SITE: 776-810 EFFECTOR-BOUND: Zn+2 sterile alpha motif NAME: sterile alpha motif SITE: 919-983 polycomb protein SCMH1 COMPARTMENT: cell nucleus MOTIF: MBT {28-126} MBT {134-235} sterile alpha motif NAME: sterile alpha motif SITE: 593-658 MOTIF: Ser phosphorylation site {S649}

References

UniProt :accession Q99496

Components

chromobox protein homolog 2 (CBX2) chromobox protein homolog 8; polycomb 3 homolog; Pc3; hPc3; rectachrome 1 (CBX8 PC3 RC1) E3 SUMO-protein ligase CBX4; chromobox protein homolog 4; polycomb 2 homolog; Pc2; hPc2 (CBX4) E3 ubiquitin-protein ligase RING1; RING finger protein 1; polycomb complex protein RING1 (RING1, RNF1) E3 ubiquitin-protein ligase RING2; Huntingtin-interacting protein 2-interacting protein 3; HIP2-interacting protein 3; protein DinG; RING finger protein 1B; RING1b; RING finger protein 2; RING finger protein BAP-1 (RNF2, BAP1, DING, HIPI3, RING1B) polycomb complex protein BMI-1; polycomb group RING finger protein 4; RING finger protein 51 (BMI1, PCGF4, RNF51) polycomb group RING finger protein 2; DNA-binding protein mel-18; RING finger protein 110; zinc finger protein 144 (PCGF2, MEL18, RNF110, ZNF144) polycomb protein SCMH1 (sex comb on midleg homolog 1, SCMH1) polyhomeotic-like protein 1 (hPH1, early development regulatory protein 1, PHC1, EDR1, PH1) polyhomeotic-like protein 2 (hPH2, early development regulatory protein 2, PHC2, EDR2, PH2) polyhomeotic-like protein 3 (hPH3, homolog of polyhomeotic 3, early development regulatory protein 3, PHC3, EDR3, PH3)