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phospholipase-A2 [PLA2] type 1 (pancreatic phospholipase-A2, snake venom phospholipase-A2, PLA2G1B)
Function:
- catalyzes the Ca+2-dependent hydrolysis of the 2- acyl groups in 3-sn-phosphoglycerides
phosphatidylcholine + H2O
1-acylglycerophosphocholine + a carboxylate
Cofactor: binds 1 Ca+2 per subunit (putative)
Structure: belongs to the phospholipase A2 family
Compartment: secreted
General
Ca+2 binding protein
phospholipase A2; phosphatidylcholine 2-acylhydrolase (PLA2)
secreted protein
Properties
SIZE: entity length = 148 aa
MW = 16 kD
COMPARTMENT: INTESTINAL-FLUID
MOTIF: signal sequence {1-15}
cysteine residue {C33}
MODIFICATION: cysteine residue {C99}
cysteine residue {C49}
MODIFICATION: cysteine residue {C146}
Ca+2-binding site
SITE: 50-50
cysteine residue {C51}
MODIFICATION: cysteine residue {C67}
Ca+2-binding site
SITE: 52-52
Ca+2-binding site
SITE: 54-54
cysteine residue {C66}
MODIFICATION: cysteine residue {C127}
cysteine residue {C67}
MODIFICATION: cysteine residue {C51}
histidine residue {H70}
Ca+2-binding site
SITE: 71-71
cysteine residue {C73}
MODIFICATION: cysteine residue {C120}
cysteine residue {C83}
MODIFICATION: cysteine residue {C113}
cysteine residue {C99}
MODIFICATION: cysteine residue {C33}
cysteine residue {C106}
MODIFICATION: cysteine residue {C118}
cysteine residue {C113}
MODIFICATION: cysteine residue {C83}
cysteine residue {C118}
MODIFICATION: cysteine residue {C106}
cysteine residue {C120}
MODIFICATION: cysteine residue {C73}
aspartate residue {D121}
cysteine residue {C127}
MODIFICATION: cysteine residue {C66}
cysteine residue {C146}
MODIFICATION: cysteine residue {C49}
SECRETED-BY: acinar cell
WITHIN: pancreas
MISC-INFO: Kd [Ca+2] 10 UM
PH[OPT] 8-10
Database Correlations
OMIM 172410
UniProt P04054
Pfam PF00068
Entrez Gene 5319
KEGG correlations
ENZYME 3.1.1.4
References
- Mayer RJ, Marshall LA.
New insights on mammalian phospholipase A2(s); comparison of
arachidonoyl-selective and -nonselective enzymes.
FASEB J. 1993 Feb 1;7(2):339-48. Review.
PMID: 8440410
- Glaser KB, Mobilio D, Chang JY, Senko N.
Phospholipase A2 enzymes: regulation and inhibition.
Trends Pharmacol Sci. 1993 Mar;14(3):92-8. Review.
PMID: 8488570