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cAMP-specific 3',5'-cyclic phosphodiesterase 4B; DPDE4; PDE32 (PDE4B, DPDE4)
Function:
- hydrolyzes the second messenger cAMP
- may be involved in mediating central nervous system effects of therapeutic agents ranging from antidepressants to antiasthmatic agents & anti-inflammatory agents
- purine metabolism; 3',5'-cyclic AMP degradation; AMP from 3',5'-cyclic AMP: step 1/1
adenosine 3',5'-cyclic phosphate + H2O = adenosine 5'-phosphate
Cofactor:
- binds 2 divalent metal cations per subunit
- site 1 may preferentially bind Zn+2
- site 2 has a preference for Mg+2 &/or Mn+2
Inhibition:
- inhibited by rolipram
Structure:
- belongs to the cyclic nucleotide phosphodiesterase family, PDE4 subfamily
Alternative splicing:
- named isoforms=3; PDE4B1, PDE4B2, PDE4B3
- additional isoforms seem to exist
Expression:
- expressed in brain, heart, lung & skeletal muscle
General
5'nucleotidase (5'-ribonucleotide phosphohydrolase)
oligomerizing protein
phosphodiesterase 4; cAMP specific phosphodiesterase (PDE4)
phosphoprotein
regulatory molecule
Properties
SIZE: entity length = 736 aa
MW = 83 kD
COMPARTMENT: cytoplasm
lysosome
plasma membrane
MOTIF: Thr phosphorylation site {T184}
Ser phosphorylation site {S272}
Tyr phosphorylation site {Y274}
cAMP-binding site
SITE: 406-410
MOTIF: histidine residue {H406}
cAMP-binding site
SITE: 447-447
cAMP-binding site
SITE: 564-564
asparagine residue {567}
cAMP-binding site
SITE: 615-615
Database Correlations
OMIM 600127
UniProt Q07343
Pfam PF00233
Entrez Gene 5142
Kegg hsa:5142
ENZYME 3.1.4.17
References
- UniProt :accession Q07343
- Entrez Gene :accession 5142
- Galoyan AA, Gurvitz BYa, Sharova NP.
Cyclic nucleotide phosphodiesterase and 5'-nucleotidase:
a coupled system.
Neurochem Res. 1989 Dec;14(12):1213-21.
PMID: 2560820