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apoptosis-stimulating of p53 protein 2; tumor suppressor p53-binding protein 2; p53-binding protein 2; p53BP2; 53BP2; Bcl2-binding protein; Bbp; renal carcinoma antigen NY-REN-51 (TP53BP2, ASPP2, BBP)

Function: - plays a role in regulation of apoptosis & cell growth - regulates TP53 by enhancing DNA binding & transactivation of TP53 on promoters of proapoptotic genes in vivo - inhibits ability of APPBP1 to conjugate NEDD8 to CUL1, & thus decreases APPBP1 ability to induce apoptosis - impedes cell cycle progression at G2/M - binds to the central domain of TP53 as well as to BCL2 - interacts with protein phosphatase 1 - interacts with RELA NF-kappa-B subunit; this interaction probably prevents activation of apoptosis, possibly by preventing its interaction with TP53 - interacts with APC2 & APPBP1 Structure: - the ankyrin repeats & the SH3 domain are required for a specific interactions with TP53 - belongs to the ASPP family - contains 2 ANK repeats - contains 1 SH3 domain Compartment: - cytoplasm, perinuclear region. nucleus - predominantly found in the perinuclear region - some small fraction is nuclear Alternative splicing: - named isoforms=2 - due to Alu sequence insertion that creates a shorter but existing form that may have an alternative function Expression: - widely expressed - expressed in spleen, thymus, prostate, testis, ovary, small intestine, colon & peripheral blood leukocyte - reduced expression in breast carcinomas expressing a wild-type TP53 protein - overexpressed in lung cancer cell lines - expressed following DNA damage induced by UV irradiation - down-regulated by wild-type, but not mutant, TP53

Interactions

molecular events

General

ankyrin repeat domain protein (ANKRD) apoptosis stimulating protein of p53; p53-binding protein (ASPP)

Properties

SIZE: entity length = 1128 aa MW = 126 kD COMPARTMENT: cytoplasm cell nucleus MOTIF: glutamine-rich region {132-173} MOTIF: glutamine residue (SEVERAL) APPBP1 interaction {332-348} Ser phosphorylation site {S480} Ser phosphorylation site {S556} Ser phosphorylation site {S569} Ser phosphorylation site {S698} proline-rich region SITE: 824-902 MOTIF: proline residue (SEVERAL) SH3-binding site NAME: SH3-binding site SITE: 866-875 Mediates interaction with APC2 {876-1128} MOTIF: ankyrin repeat NAME: ankyrin repeat SITE: 958-990 ankyrin repeat NAME: ankyrin repeat SITE: 991-1023 src homology 3 [SH3] domain SITE: 1057-1119

Database Correlations

OMIM 602143 UniProt Q13625 PFAM correlations Entrez Gene 7159 Kegg hsa:7159

References

  1. UniProt :accession Q13625
  2. Samuels-Lev Y et al. ASPP proteins specifically stimulate the apoptotic function of p53. Molecular Cell 8:781-94. 2001 PMID: 11684014
  3. Entrez Gene :accession 7159

Component-of

molecular complex