tumor suppressor p53-binding protein 1; p53-binding protein 1; p53BP1; 53BP1 (TP53BP1)

Function: - may have a role in checkpoint signaling during mitosis - enhances TP53-mediated transcriptional activation. - plays a role in the response to DNA damage - phosphorylated at basal level in the absence of DNA damage - hyper-phosphorylated (ATM-dependent) in response to DNA damage induced by ionizing radiation - hyper-phosphorylated in (ATR-dependent) in response to DNA damage induced by UV irradiation - interacts with IFI202A (putative) - binds to the central domain of TP53/p53. - interacts with DCLRE1C - interacts with histone H2AFX; this requires phosphorylation of H2AFX on Ser-139 - interacts with histone H4 dimethylated at Lys-20 - low affinity for histone H4 containing monomethylated Lys-20 - does not bind histone H4 containing unmethylated or trimethylated Lys-20 - low affinity for histone H3 that has been dimethylated on Lys-79 - very low affinity for histone H3 monomethylated on Lys-79 (in vitro) - does not bind unmethylated histone H3. - interacts with MUM1/EXPAND1 Structure: - may form homo-oligomers - asymmetrically dimethylated on Arg residues by PRMT1 - methylation is required for DNA binding - contains 2 BRCT domains Compartment: - nucleus - kinetochore - associated with kinetochores - both nuclear & cytoplasmic in some cells - recruited to sites of DNA damage, such as double stand breaks - methylation of histone H4 at Lys-20 is required for efficient localization to double strand breaks Alternative splicing: named isoforms=2

Properties

SIZE: entity length = 1972 aa MW = 214 kD COMPARTMENT: cytoplasm cell nucleus MOTIF: Ser phosphorylation site {S105} Ser phosphorylation site {S166} Ser phosphorylation site {S176} Ser phosphorylation site {S178} Ser phosphorylation site {S222} Ser phosphorylation site {S265} Ser phosphorylation site {S294} Thr phosphorylation site {T302} Ser phosphorylation site {S316} Ser phosphorylation site {S366} Ser phosphorylation site {S380} Thr phosphorylation site {T382} Thr phosphorylation site {T394} Ser phosphorylation site {S395} Ser phosphorylation site {S398} Ser phosphorylation site {S452} Ser phosphorylation site {S500} Tyr phosphorylation site {Y522} Ser phosphorylation site {S523} Ser phosphorylation site {S525} Thr phosphorylation site {T543} Thr phosphorylation site {T548} Ser phosphorylation site {S552} Ser phosphorylation site {S566} Ser phosphorylation site {S580} Ser phosphorylation site {S625} Ser phosphorylation site {S635} Ser phosphorylation site {S639} Ser phosphorylation site {S640} Ser phosphorylation site {S659} Ser phosphorylation site {S660} Ser phosphorylation site {S662} Thr phosphorylation site {T750} Ser phosphorylation site {S751} Ser phosphorylation site {S754} Ser phosphorylation site {S755} Ser phosphorylation site {S771} Ser phosphorylation site {S782} Ser phosphorylation site {S784} Ser phosphorylation site {S809} Ser phosphorylation site {S831} Ser phosphorylation site {S834} Thr phosphorylation site {T855} Ser phosphorylation site {S876} Ser phosphorylation site {S877} Ser phosphorylation site {S892} Ser phosphorylation site {S993} Thr phosphorylation site {T996} Ser phosphorylation site {S999} Ser phosphorylation site {S1002} Thr phosphorylation site {T1020} Ser phosphorylation site {S1028} Thr phosphorylation site {T1055} Thr phosphorylation site {T1056} Ser phosphorylation site {S1068} Ser phosphorylation site {S1094} Ser phosphorylation site {S1101} Ser phosphorylation site {S1104} Ser phosphorylation site {S1113} Ser phosphorylation site {S1114} Thr phosphorylation site {T1171} Thr phosphorylation site {T1214} Ser phosphorylation site {S1216} Ser phosphorylation site {S1219} Ser phosphorylation site {S1290} Ser phosphorylation site {S1316} Ser phosphorylation site {S1317} Ser phosphorylation site {S1320} Ser phosphorylation site {S1362} Ser phosphorylation site {S1368} Thr phosphorylation site {T1372} GAR {1396-1403} Ser phosphorylation site {S1426} Ser phosphorylation site {S1430} Ser phosphorylation site {S1435} Ser phosphorylation site {S1460} Ser phosphorylation site {S1462} Ser phosphorylation site {S1474} dimethylated histone H4 interaction {1495-1523} Thr phosphorylation site {T1609} Ser phosphorylation site {S1618} Ser phosphorylation site {S1631} Ser phosphorylation site {S1634} Ser phosphorylation site {S1635} Thr phosphorylation site {T1638} Thr phosphorylation site {T1640} serine-rich region {1642-1646} MOTIF: serine residue (SEVERAL) Ser phosphorylation site {S1643} Ser phosphorylation site {S1645} Ser phosphorylation site {S1646} Thr phosphorylation site {T1647} Thr phosphorylation site {T1648} Thr phosphorylation site {T1672} Ser phosphorylation site {S1673} Ser phosphorylation site {S1678} Ser phosphorylation site {S1701} Ser phosphorylation site {S1705} Thr phosphorylation site {T1709} BRCA1 C-terminal (BRCT) motif SITE: 1724-1848 MOTIF: glutamate-rich region {1760-1764} MOTIF: glutamate residue (SEVERAL) BRCA1 C-terminal (BRCT) motif SITE: 1864-1964

Database Correlations

OMIM 605230 UniProt Q12888 Pfam PF09038 Entrez Gene 7158 Kegg hsa:7158