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origin of replication
origins of replication are typically assigned names containing ori
Function:
- sequence in a genome at which replication is initiated
- binds origin of replication complex which, with help from two further protein factors (Cdc6 & Cdt1), load the minichromosome maintenance protein complex
- once assembled, pre-replication complex unwinds, & begins to copy DNA
- DNA replication may proceed from this point bidirectionally or unidirectionally
- eukaryotes often have multiple origins of replication on each linear chromosome, with up to 100,000 present in a single human cell [2]
- many origins of replication shortens time of DNA replication
- the segment of DNA that is copied starting from each unique replication origin is called a replicon
Structure:
- AT rich DNA
- DNA sequences at the replication origins vary
- despite this sequence variation, all the origins form a base for assembly of the origin of replication complex
Related
origin of replication complex (origin recognition complex, ORC)
replicon
General
biochemistry
References
- WikiPedia: Origin of Replication
http://en.wikipedia.org/wiki/Origin_of_replication
- Nasheuer HP, Smith R, Bauerschmidt C, Grosse F,
Weisshart K
Initiation of eukaryotic DNA replication: regulation and
mechanisms.
Prog. Nucleic Acid Res. Mol. Biol. (2002) 72: 41-94.
PMID: 12206458