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oligonucleotide ligation assay

Indications: - discrimination of SNP alleles Procedure: - uses pairs of ligation probes - ligation implies hybridization of each ligation probe in the correct alignment to the template of interest - one of the ligation probes is covalently linked to biotin, the other with a detectable function

General

molecular diagnostic test

References

  1. Jarvius J, Nilddon M, Landegren U Chapter 15. Oligonucleotide ligation Assay Methods in Molecular Biology. vol 212 Single Nucleotide Polymorphisms: Methods and Protocols. Kwok PY (ed). Humana Press Inc. Totowa NJ