obscurin; obscurin-RhoGEF; obscurin-myosin light chain kinase; obscurin-MLCK (OBSCN, KIAA1556, KIAA1639)

Function: - role in myofibrillogenesis - role in assembly of myosin into sarcomeric A bands in striated muscle - isoform 3 together with ANK1 isoform Mu17/ank1.5 may provide a molecular link between the sarcoplasmic reticulum & myofibrils - isoform 3 interacts with TTN/titin, ANK1 isoform Mu17/ank1.5, & with calmodulin (Ca⁺²-independent) - associates with fast skeletal muscle myosin (putative) Cofactor: Mg⁺² (putative) Structure: - belongs to the protein kinase superfamily, CAMK Ser/Thr protein kinase family - contains 1 DH (DBL-homology) domain - contains 3 fibronectin F3 modules - contains 55 Ig-like (immunoglobulin-like) domains - contains 1 IQ domain - contains 2 PH domains - contains 2 protein kinase domains - contains 1 SH3 domain Compartment: - isoform 3: cytoplasm, myofibril, sarcomere, M line - cytoplasm, myofibril, sarcomere, Z line - in differentiating skeletal muscle cells, isoform 3 primarily localizes to the sarcomeric M-line & less frequently to the Z-disk - isoform 3 colocalizes with ANK1 isoform Mu17/ank1.5 at the M-line in differentiated skeletal muscle cells Alternative splicing: - named isoforms=5 - additional isoforms seem to exist Note: - isoform 3 lacks the kinase domain

General

cytoskeletal protein phosphoprotein

Properties

SIZE: entity length = 7968 aa MW = 868 kD COMPARTMENT: cytoplasm MOTIF: immunoglobulin superfamily domain {10-7552} (55) MOTIF: cysteine residue {C31} MODIFICATION: cysteine residue {C82} cysteine residue {C82} MODIFICATION: cysteine residue {C31} fibronectin type III domain or F3 module {512-4614} (3) glutamine-rich region {4846-4849} MOTIF: glutamine residue (SEVERAL) IQ motif {4872-4901} src homology 3 [SH3] domain SITE: 5601-5668 Dbl homology domain {5693-5877} PH domain {5895-6004} kinase domain SITE: 6468-6721 MOTIF: ATP-binding site NAME: ATP-binding site SITE: 6474-6482 ATP-binding site NAME: ATP-binding site SITE: 6497-6497 aspartate residue {D6587} serine-rich region {6789-6796} MOTIF: serine residue (SEVERAL) proline-rich region SITE: 6969-7117 MOTIF: proline residue (SEVERAL) fibronectin type III domain or F3 module {7557-7644} Thr phosphorylation site {T7671} kinase domain SITE: 7672-7924 MOTIF: ATP-binding site NAME: ATP-binding site SITE: 7678-7686 ATP-binding site NAME: ATP-binding site SITE: 7701-7701 aspartate residue {D7791}

Database Correlations

OMIM 608616 UniProt Q5VST9 PFAM correlations Entrez Gene 84033 Kegg hsa:84033 ENZYME 2.7.11.1

References

UniProt :accession Q5VST9