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N-acetylgalactosamine 4-sulfate 6-O-sulfotransferase; GalNAc4S-6ST; B-cell RAG-associated gene protein; hBRAG, (GALNAC4S6ST, BRAG, CHST15, KIAA0598)
Function:
1) sulfotransferase
2) transfers sulfate from 3'-phosphoadenosine 5'-phosphosulfate (PAPS) to the C-6 hydroxyl group of the GalNAc 4-sulfate residue of chondroitin sulfate A & forms chondroitin sulfate E containing GlcA-GalNAc(4,6-SO4) repeating units
3) transfers sulfate to a unique nonreducing terminal sequence, GalNAc(4SO4)-GlcA(2SO4)-GalNAc(6SO4), to yield a highly sulfated structure similar to the structure found in thrombomodulin chondroitin sulfate
4) B-cell receptor involved in BCR ligation-mediated early activation & mediates regulatory signals key to B-cell development &/or regulate of B-cell-specific RAG expression (putative)
5) interacts with phosphorylated proteins in resting B-cells, including HCK (putative)
6) inhibited by phenyl beta-GalNAc(4,6-SO4)
3'-phosphoadenylyl sulfate + dermatan
adenosine 3',5'-bisphosphate + dermatan 6'-sulfate
3'-phosphoadenylyl sulfate + chondroitin
adenosine 3',5'-bisphosphate + chondroitin 6'-sulfate
Cofactors:
1) divalent metal cations
2) glutathione
Structure:
1) homodimer, disulfide-linked
2) glycosylated
Compartment:
- Golgi membrane, cell surface (minor)
- colocalizes with RAG1 in pro-B, pre-B, & mature B-cell lines
Alternative splicing: named isoforms=2
Expression:
- expressed in B-cell-enriched tissues, including fetal & adult spleen, lymph node, tonsil, bone marrow & peripheral leukocytes
- not expressed in fetal or adult thymus, T-cells
General
glycoprotein
membrane protein
sulfotransferase
Properties
SIZE: MW = 65 kD
entity length = 561 aa
COMPARTMENT: plasma membrane
golgi
MOTIF: transmembrane domain {81-101}
N-glycosylation site {N364}
Database Correlations
OMIM 608277
UniProt Q7LFX5
Pfam PF00685
ENZYME 2.8.2.33
References
- UniProt :accession Q7LFX5
- GGDB, GlycoGene database
http://ggdb.muse.aist.go.jp/GGDB/index.jsp