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nucleoside diphosphate kinase-B (NDP kinase B, c-myc purine-binding transcription factor PUF, nm23-H2, NME2)

Function: - major role in the synthesis of nucleoside triphosphates other than ATP - negatively regulates Rho activity by interacting with AKAP13/LBC - acts as a transcriptional activator of the c-Myc gene - binds DNA nonspecifically - N-terminus is blocked ATP + nucleoside diphosphate ADP + nucleoside triphosphate Cofactor: Mg+2 (putative) Structure: - hexamer of two different chains: A & B (A6, A5B, A4B2, A3B3, A2B4, AB5, B6). interacts with AKAP13 - belongs to the NDK family Compartment: cytoplasm. nucleus Pathology: - found in diminished amount in tumor cells of high metastatic potential

General

DNA-binding protein nucleoside diphosphate kinase (NDK) or NDP kinase transcription factor (TF)

Properties

CONFIGURATION: hexamer SIZE: entity length = 152 aa MW = 17 kD COMPARTMENT: cytoplasm cell nucleus MOTIF: AKAP13 interaction {1-66} MOTIF: ATP-binding site NAME: ATP-binding site SITE: 12-12 ATP-binding site NAME: ATP-binding site SITE: 60-60 ATP-binding site NAME: ATP-binding site SITE: 88-88 ATP-binding site NAME: ATP-binding site SITE: 94-94 ATP-binding site NAME: ATP-binding site SITE: 105-105 ATP-binding site NAME: ATP-binding site SITE: 115-115 histidine residue {H118}

Database Correlations

OMIM 156491 UniProt P22392 Pfam PF00334 ENZYME 2.7.4.6

References

  1. Postel EH, Berberich SJ, Flint SJ, Ferrone CA. Human c-myc transcription factor PuF identified as nm23-H2 nucleoside diphosphate kinase, a candidate suppressor of tumor metastasis. Science. 1993 Jul 23;261(5120):478-80. PMID: 8392752
  2. UniProt :accession P22392