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nucleoside diphosphate kinase-A, (NDP kinase A, tumor metastatic process-associated protein, metastasis inhibition factor nm23, nm23-H1, NME1, nm23/H1 nucleoside diphosphate kinase, granzyme A-activated DNase, GAAD)

Function: - major role in the synthesis of nucleoside triphosphates other than ATP - potential negative regulator of cancer metastasis - interacts with SET - N-terminus is blocked ATP + nucleoside diphosphate ADP + nucleoside triphosphate Cofactor: Mg+2 Structure: - hexamer of two different chains: A & B (A6, A5B, A4B2, A3B3, A2B4, AB5, B6) - belongs to the NDK family Compartment: cytoplasm, nucleus Pathology: - expressed in lung carcinoma cell lines but not in normal lung tissues - found in diminished amount in tumor cells of high metastatic potential - somatic mutations of NME1 are found in neuroblastoma increased NME1 in neuroblastoma is correlated with features of the disease that are associated with aggressive tumors; may thus have distinct if not opposite roles in different tumors

General

DNA-binding protein nucleoside diphosphate kinase (NDK) or NDP kinase transcription factor (TF)

Properties

CONFIGURATION: hexamer SIZE: entity length = 152 aa MW = 17 kD COMPARTMENT: cytoplasm cell nucleus MOTIF: ATP-binding site NAME: ATP-binding site SITE: 12-12 ATP-binding site NAME: ATP-binding site SITE: 60-60 ATP-binding site NAME: ATP-binding site SITE: 88-88 ATP-binding site NAME: ATP-binding site SITE: 94-94 ATP-binding site NAME: ATP-binding site SITE: 105-105 ATP-binding site NAME: ATP-binding site SITE: 115-115 histidine residue {H118}

Database Correlations

OMIM 156490 UniProt P15531 Pfam PF00334 ENZYME 2.7.4.6

References

  1. Postel EH, Berberich SJ, Flint SJ, Ferrone CA. Human c-myc transcription factor PuF identified as nm23-H2 nucleoside diphosphate kinase, a candidate suppressor of tumor metastasis. Science. 1993 Jul 23;261(5120):478-80. PMID: 8392752
  2. UniProt :accession P15531

Component-of

SET complex