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nucleolin; protein C23 (NCL)

Function: - nucleolin is the major nucleolar protein of growing eukaryotic cells - it is found associated with intranucleolar chromatin & pre-ribosomal particles - it induces chromatin decondensation by binding to histone H1 - it is thought to play a role in pre-rRNA transcription & ribosome assembly - may play a role in the process of transcriptional elongation - binds RNA oligonucleotides with 5'-UUAGGG-3' repeats more tightly than the telomeric single-stranded DNA 5'-TTAGGG-3' repeats - interacts with APTX & NSUN2 - component of the SWAP complex - component of a complex which is at least composed of HTATSF1/Tat-SF1, the P-TEFb complex components CDK9 & CCNT1, RNA polymerase 2, SUPT5H, & NCL/nucleolin Structure: contains 4 RRM domain (RNA recognition motif)s Compartment: nucleus, nucleolus

Interactions

molecular events

Specific

N-60

General

DNA-binding protein phosphoprotein RNA-binding protein

Properties

SIZE: entity length = 710 aa MW = 77 kD COMPARTMENT: cell nucleus MOTIF: Ser phosphorylation site {S28} Ser phosphorylation site {S34} Ser phosphorylation site {S41} Ser phosphorylation site {S42} 8 X 8 AA tandem repeats of X-T-P-X-K-K-X- X {58-135} MOTIF: consensus repeat {58-65} Ser phosphorylation site {S67} Thr phosphorylation site {T69} consensus repeat {75-82} Thr phosphorylation site {T76} consensus repeat {83-90} consensus repeat {91-98} consensus repeat {99-104} Thr phosphorylation site {T99} consensus repeat {105-112} Thr phosphorylation site {T105} consensus repeat {120-127} Thr phosphorylation site {T121} consensus repeat {128-135} acidic region {143-171} MOTIF: acidic residue (SEVERAL) Ser phosphorylation site {S145} Ser phosphorylation site {S153} Ser phosphorylation site {S184} acidic region {185-209} MOTIF: acidic residue (SEVERAL) Ser phosphorylation site {S206} Thr phosphorylation site {T214} acidic region {234-271} MOTIF: acidic residue (SEVERAL) RNP motif NAME: RNP motif SITE: 307-383 FOR-BINDING-OF: ribonucleic acid MOTIF: ribonucleoprotein-1 motif NAME: ribonucleoprotein-1 motif FOR-BINDING-OF: ribonucleic acid MOTIF: ribonucleoprotein-1 motif ribonucleoprotein-2 motif ribonucleoprotein-2 motif FOR-BINDING-OF: ribonucleic acid MOTIF: ribonucleoprotein-1 motif ribonucleoprotein-2 motif Thr phosphorylation site {T367} RNP motif NAME: RNP motif SITE: 393-466 FOR-BINDING-OF: ribonucleic acid MOTIF: ribonucleoprotein-1 motif NAME: ribonucleoprotein-1 motif FOR-BINDING-OF: ribonucleic acid MOTIF: ribonucleoprotein-1 motif ribonucleoprotein-2 motif ribonucleoprotein-2 motif FOR-BINDING-OF: ribonucleic acid MOTIF: ribonucleoprotein-1 motif ribonucleoprotein-2 motif RNP motif NAME: RNP motif SITE: 486-560 FOR-BINDING-OF: ribonucleic acid MOTIF: ribonucleoprotein-1 motif NAME: ribonucleoprotein-1 motif FOR-BINDING-OF: ribonucleic acid MOTIF: ribonucleoprotein-1 motif ribonucleoprotein-2 motif ribonucleoprotein-2 motif FOR-BINDING-OF: ribonucleic acid MOTIF: ribonucleoprotein-1 motif ribonucleoprotein-2 motif Ser phosphorylation site {S563} RNP motif NAME: RNP motif SITE: 572-647 FOR-BINDING-OF: ribonucleic acid MOTIF: ribonucleoprotein-1 motif NAME: ribonucleoprotein-1 motif FOR-BINDING-OF: ribonucleic acid MOTIF: ribonucleoprotein-1 motif ribonucleoprotein-2 motif ribonucleoprotein-2 motif FOR-BINDING-OF: ribonucleic acid MOTIF: ribonucleoprotein-1 motif ribonucleoprotein-2 motif Ser phosphorylation site {S580} Ser phosphorylation site {S619}

Database Correlations

OMIM 164035 UniProt P19338 PFAM correlations Entrez Gene 4691 Kegg hsa:4691

References

  1. UniProt :accession P19338
  2. Jordan G At the heart of the nucleolus. Nature 329:489 1987 PMID: 3309675
  3. Ginisty H et al Structure and functions of nucleolin. J Cell Sci. 1999 Mar;112 ( Pt 6):761-72. PMID: 10036227

Component-of

molecular complex