nucleolar GTP-binding protein 3; guanine nucleotide-binding protein-like 3; E2-induced gene 3 protein; novel nucleolar protein 47; NNP47; nucleolar GTP-binding protein 3; nucleostemin (GNL3, E2IG3, NS)

Function: - implicated in proliferative capacity of stem cells - may interact with p53 via basic region Structure: - the basic domain (B) allows nucleolar localization in the absence of GTP - the intermediate domain (I) inhibits nucleolar localization by the B domain & is required for exit from the nucleolus - exit from the nucleolus to the nucleoplasm requires both the I & the acidic (A) domains, & may be triggered by GTP hydrolysis (putative) - in contrast to other GTP-binding proteins, this family is characterized by a circular permutation of the GTPase motifs described by a G4-G1-G3 pattern - belongs to the MMR1/HSR1 GTP-binding protein family - contains 1 G (guanine nucleotide-binding) domain Compartment: shuttles between the nucleus & nucleolus Alternative splicing: named isoforms=2 Pathology: - may play a role in tumorigenesis - increased expression in at least some lung cancers

General

guanine nucleotide regulatory protein (GNRP) nuclear protein phosphoprotein

Properties

SIZE: entity length = 549 aa MW = 62 kD COMPARTMENT: cell nucleus MOTIF: basic region {2-46} coiled coil {56-95} GTP-binding site SITE: 178-181 GTP-binding site SITE: 261-268 G {266-347} Intermediate {282-456} MOTIF: GTP-binding site SITE: 305-308 Acidic {465-543} MOTIF: Ser phosphorylation site {S529}

Database Correlations

OMIM 608011 UniProt Q9BVP2 PFAM correlations Entrez Gene 26354 Kegg hsa:26354

References

UniProt :accession Q9BVP2