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nucleobindin-1; CALNUC (NUCB1, NUC)
Function:
- major Ca+2-binding protein of the Golgi
- may have a role in Ca+2 homeostasis (putative)
- phosphorylated upon DNA damage, probably by ATM or ATR
- interacts with GNAI2 & GNAI3
Structure:
- the EF-hand domains are unfolded in the absence of Ca+2 & fold upon Ca+2 addition
- belongs to the nucleobindin family
- contains 2 EF-hand domains
Compartment:
- Golgi, cis-Golgi network membrane
- peripheral membrane protein; lumenal side (putative)
- cytoplasm (putative)
- small fraction of the protein may be cytoplasmic
Expression:
- expressed both in fetal & adult heart, lung, liver, kidney & brain, & in adult skeletal muscle, placenta & pancreas
Note:
- discovered as DNA-binding protein in the serum of lupus-prone mice
General
Ca+2 binding protein
DNA-binding protein
phosphoprotein
Properties
SIZE: entity length = 461 aa
MW = 54 kD
COMPARTMENT: cytoplasm
golgi
MOTIF: signal sequence {1-26}
Ser phosphorylation site {S82}
Ser phosphorylation site {S86}
coiled coil {150-218}
MOTIF: DNA-binding motif
SITE: 172-218
Tyr phosphorylation site {Y179}
Ser phosphorylation site {S224}
Binds to GNAI2 and GNAI3 {228-321}
MOTIF: EF hand
SITE: 240-275
Ca+2-binding site
SITE: 253-264
EF hand
SITE: 292-327
MOTIF: Ca+2-binding site
SITE: 305-316
coiled coil {341-407}
MOTIF: Ser phosphorylation site {S369}
Thr phosphorylation site {T372}
glutamine-rich region {401-407}
MOTIF: glutamine residue (SEVERAL)
Database Correlations
OMIM 601323
UniProt Q02818
Entrez Gene 4924
Kegg hsa:4924
References
UniProt :accession Q02818