nuclear RNA export factor 1; Tip-associated protein; Tip-associating protein; mRNA export factor TAP (NXF1, TAP)

Function: - nuclear export of mRNA with retroviral constitutive transport elements - export of mRNA from the nucleus to the cytoplasm - heterodimer with NXT1 - interacts via NTF2 domain - the NXF1-NXT1 heterodimer is involved in the export of HSP70 mRNA in conjunction with ALYREF/THOC4 & THOC5 - formation of NXF1/NXT1 heterodimers is required for NXF1-mediated nuclear mRNA export - interacts with NXT2, E1B-AP5, RAE1, THOC4, nucleoporins - component of exon junction complex - interacts with FYTTD1/UIF, EIF4A3, NUPL2 & THOC5 - interacts with: Saimiriine herpesvirus 2 TIP protein, NUPL2 Structure: - minimal CTE binding domain consists of an RNP-type RNA binding domain (RBD) & leucine-rich repeats - the nucleoporin binding domain consists of a NTF2 domain & a TAP-C domain (UBA-like domain) - 2 nucleoporin-FG-repeats binding sites (one in the NTF2 & the other in the TAP-C domain) which contribute to nucleoporin association & act synergistically to export cellular mRNAs - the NTF2 domain is functional only in the presence of NXT1 & is essential for the export of mRNA from the nucleus - the TAP-C domain mediates direct interactions with nucleoporin-FG-repeats & is necessary & sufficient for localization of NXF1 to the nuclear rim - the conserved loop 594-NWD-596 of the TAP-C domain has a critical role in the interaction with nucleoporins - the leucine-rich repeats are essential for the export of mRNA from the nucleus - the RNA-binding domain is a non-canonical RNP-type domain - belongs to the NXF family - contains 4 LRR repeats (leucine-rich repeats) - contains 1 NTF2 domain - contains 1 RRM domain (RNA recognition motif) - contains 1 TAP-C domain Compartment: - nuclear, nucleoplasm & nuclear speckle - localized predominantly in the nucleoplasm & at both the nucleoplasmic & cytoplasmic faces of the nuclear pore complex - cytoplasm - travels to the cytoplasm as part of the exon junction complex bound to mRNA - shuttles between nucleus & cytoplasm Alternative splicing: named isoforms=2 Expression: expressed ubiquitously

Properties

SIZE: entity length = 619 aa MW = 70 kD COMPARTMENT: cytoplasm cell nucleus MOTIF: ALYREF/THOC4 interaction {2-371} acetylation site SITE: N-TERMINUS EFFECTOR-BOUND: acetyl nuclear translocation signal {67-100} nuclear export signal {83-110} RNP motif NAME: RNP motif SITE: 119-198 FOR-BINDING-OF: ribonucleic acid MOTIF: ribonucleoprotein-1 motif NAME: ribonucleoprotein-1 motif FOR-BINDING-OF: ribonucleic acid MOTIF: ribonucleoprotein-1 motif ribonucleoprotein-2 motif ribonucleoprotein-2 motif FOR-BINDING-OF: ribonucleic acid MOTIF: ribonucleoprotein-1 motif ribonucleoprotein-2 motif leucine-rich repeat SITE: 266-291 MOTIF: leucine residue (SEVERAL) leucine-rich repeat SITE: 292-315 MOTIF: leucine residue (SEVERAL) leucine-rich repeat SITE: 316-343 MOTIF: leucine residue (SEVERAL) leucine-rich repeat SITE: 344-371 MOTIF: leucine residue (SEVERAL) NTF2 {386-536} proline-rich region SITE: 551-561 MOTIF: proline residue (SEVERAL) TAP-C {565-619}

Database Correlations

OMIM 602647 UniProt Q9UBU9 PFAM correlations Entrez Gene 10482 Kegg hsa:10482

References

UniProt :accession Q9UBU9