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carboxy-terminal domain RNA polymerase II polypeptide A small phosphatase 1; nuclear LIM interactor-interacting factor 3; NLI-IF; NLI-interacting factor 3; small C-terminal domain phosphatase 1; SCP1; small CTD phosphatase 1 (CTDSP1, NIF3, NLIIF, SCP1)
Function:
- preferentially catalyzes dephosphorylation of Ser-5 within the tandem 7 residues repeats in the C-terminal domain (CTD) of the largest RNA polymerase 2 subunit POLR2A
- negatively regulates RNA polymerase 2 transcription, possibly by controlling transition from initiation/capping to processive transcript elongation
- recruited by REST to neuronal genes that contain RE-1 elements, leading to neuronal gene silencing in non-neuronal cells
- stimulated by GTF2F1
- inhibited by beryllofluoride anions
- interacts with GTF2F1
- interacts with REST
pH dependence: optimum pH is 5
Cofactor: binds 1 Mg+2 per monomer
Structure:
- monomer (putative).
- contains 1 FCP1 homology domain
Compartment:
- nucleus
- colocalizes with RNA polymerase 2
Alternative splicing: named isoforms=2
Expression:
- expression is restricted to non-neuronal tissues
- highest expression in skeletal muscle, spleen, lung & placenta
Related
LIM domain
LIM domain protein kinase
General
nuclear LIM interactor-interacting factor (NLI-interacting factor)
Properties
SIZE: entity length = 261 aa
MW = 29 kD
COMPARTMENT: cell nucleus
STATE: active state
MOTIF: FCP1 homology {86-244}
MOTIF: aspartate residue {D96}
Mg+2-binding site
SITE: 96-96
aspartate residue {D98}
Mg+2-binding site
SITE: 98-98
threonine residue {152}
lysine residue {190}
Mg+2-binding site
SITE: 207-207
Database Correlations
OMIM 605323
UniProt Q9GZU7
Pfam PF03031
Entrez Gene 58190
Kegg hsa:58190
ENZYME 3.1.3.16
References
UniProt :accession Q9GZU7