nuclear distribution protein nudE homolog 1 (NudE, NDE1, NUDE)

Function: - required for centrosome duplication & formation & function of the mitotic spindle - essential for development of the cerebral cortex - may regulate production of neurons by controlling orientation of the mitotic spindle during division of cortical neuronal progenitors of the proliferative ventricular zone in brain - orientation of the division plane perpendicular to layers of the cortex gives rise to two proliferative neuronal progenitors whereas parallel orientation of the division plane yields one proliferative neuronal progenitor & a post- mitotic neuron - a premature shift towards a neuronal fate within the progenitor population may result in an overall reduction in the final number of neurons & an increase in the number of neurons in the deeper layers of the cortex (putative) - self-associates - interacts with CENPF, CEP110, dynein, SLMAP & TCP1 (putative) - interacts with dynactin, tubulin gamma, PAFAH1B1, PCM1 & PCNT - phosphorylated in mitosis Structure: belongs to the nudE family Compartment: - cytoplasm, centrosome, kinetochore, spindle - concentrates at plus ends of microtubules coincident with kinetochores in metaphase & anaphase - localizes to cleavage furrow during cytokinesis (putative) - localizes to interphase centrosome & to mitotic spindle Alternative splicing: named isoforms=2

General

cytoskeletal protein phosphoprotein

Properties

SIZE: MW = 39 kD entity length = 346 aa COMPARTMENT: cytoplasm MOTIF: Self-association {1-93} coiled coil {18-188} MOTIF: PAFAH1B1 interaction {88-156} CENPF interaction {167-290} MOTIF: Thr phosphorylation site {T246} Tyr phosphorylation site {Y279} Ser phosphorylation site {S282}

Database Correlations

UniProt Q9NXR1 Pfam PF04880

References

UniProt :accession Q9NXR1