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nuclear body (nuclear domain 10, PML oncogenic domain, POD, nuclear dot, KR body)

Function: - seeems to be involved in transcriptional regulation - DAXX seems to regulate transcription in PML nuclear bodies together with transcription factor PML & may influence TNFRSF6-dependent apoptosis - role in checkpoint responses upon DNA damage [3] - heterochromatin remodelling in G2 phase [4] Structure: - diameters between 0.2 & 1 mm - number & size change during the cell cycle - electron microscopic analysis show that the nuclear body is shaped like a doughnut - components of the nuclear body include: - transcription factor PML - sumoylation is necessary for PML nuclear body formation - nuclear body protein SP140 - RB protein - protein p53 - CBP - DNA helicase BLM22 - eIF-4 - INT-6 - Daxx - SUMO-1 - HAUSP - Sp100 Expression: - cells typically contain 10-30 nuclear bodies per nucleus Notes: - discrete nuclear speckles containing transcription factor PML along with other proteins, which were originally identified as autoantigens in primary biliary cirrhosis patients

Related

nuclear body (GO:0016604)

General

intracellular compartment (intracellular space)

Properties

References

  1. Zhong S et al The transcriptional role of PML and the nuclear body Nature Cell Biology 2000 2:E85 http://www.math.mcgill.ca/~dstephens/Presentations/PMLPresentation/PML-bodies.NatCB00.pdf
  2. Shen TH et al The Mechanisms of PML-Nuclear Body Formation Mol Cell. 2006 November 3; 24(3): 331-339. http://www.ncbi.nlm.nih.gov/pmc/articles/PMC1978182/
  3. Bernardi R and Pandolfi P Role of PML and the PML-nuclear body in the control of programmed cell death Oncogene (2003) 22, 9048-9057 http://www.nature.com/onc/journal/v22/n56/abs/1207106a.html
  4. Luciani JJ PML nuclear bodies are highly organised DNA-protein structures with a function in heterochromatin remodelling at the G2 phase Journal of Cell Science 119, 2518-2531 (2006) http://jcs.biologists.org/cgi/content/short/119/12/2518
  5. van Damme E A manually curated network of the PML nuclear body interactome reveals an important role for PML-NBs in SUMOylation dynamics. Int J Biol Sci 2010; 6:51-67 http://www.biolsci.org/v06p0051.htm
  6. UniProt :accession Q13342 UniProt :accession P23497

Component-of

cell nucleus