NSFL1 cofactor p47 (p97 cofactor p47, NSFL1C)

Function: 1) decreased ATPase activity of VCP 2) necessary for fragmentation of Golgi stacks during mitosis & for VCP-mediated reassembly of Golgi stacks after mitosis 3) role in VCP-mediated formation of transitional endoplasmic reticulum (putative) 4) component of ternary complex containing STX5A, NSFL1C & VCP 5) forms homotrimer that binds to one end of VCP homohexamer - the complex binds to membranes enriched in phosphatidylethanolamine-containing lipids - interaction with VCIP135 leads to dissociation of the complex via ATP hydrolysis by VCP 6) binds ubiquitin & mono-ubiquitinated proteins via its N-terminal UBA-like domain when bound to VCP 7) phosphorylated during mitosis - phosphorylation inhibits interaction with Golgi membranes & is required for fragmentation of the Golgi stacks during mitosis Structure: contains 1 UBX domain Compartment: - nucleus, Golgi - predominantly nuclear in interphase - bound to the axial elements of sex chromosomes in pachytene spermatocytes - small proportion is associated with Golgi stacks Alternative splicing: named isoforms=3

General

nuclear protein phosphoprotein

Properties

SIZE: MW = 41 kD entity length = 370 aa COMPARTMENT: cytoplasm cell nucleus golgi MOTIF: glutamate-rich region {84-90} MOTIF: glutamate residue (SEVERAL) nuclear translocation signal {109-115} MOTIF: Ser phosphorylation site {S114} Ser phosphorylation site {S140} FOR-PHOSPHORYLATION-BY: cyclin-dependent kinase-1 nuclear translocation signal {172-175} Ser phosphorylation site {S176} Ser phosphorylation site {S272} UBX {291-368}

Database Correlations

OMIM 606610 UniProt Q9UNZ2 PFAM correlations

References

UniProt :accession Q9UNZ2