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NIRVANA
NIRVANA stands for Nanopore sequencing of Isothermal Rapid Viral Amplification for Near real-time Analysis
Indications:
- can simultaneously detect & sequence SARS-CoV2, influenza & other viruses
Procedure:
- uses small, portable device that can screen 96 samples at the same time
- simultaneously test samples for COVID-19, influenza A, human adenovirus, & non-SARS-CoV2 human coronavirus
- including sequences of 5 regions of SARS-CoV2 that captures the B.1.1.7 variant
- within 15 minutes, the device begins to report positive & negative results
- within 3 hours, the device finalizes results of 96 samples
Mechanism of action:
- uses isothermal recombinase polymerase amplification (RPA) coupled with real-time nanopore sequencing
- allows for amplifying longer stretches of DNA, & to probe for multiple genes at the same time
Notes:
- portable, pocket-sized machine
Related
SARS Cov2 laboratory
General
SARS CoV2/influenza virus in specimen
point of care test
References
- Salk Institute
Fast, portable test can diagnose COVID-19 and track variants.
The field test, called NIRVANA, can simultaneously detect and
sequence SARS-CoV-2, influenza and other viruses.
Science News. March 31, 2021
https://www.sciencedaily.com/releases/2021/03/210331173739.htm
- Bi C, Ramos-Mandujano G, Tian Y et al
Simultaneous Detection and Mutation Surveillance of SARS-CoV-2 and co-infections
of multiple respiratory viruses by Rapid field-deployable sequencing.
Med. 2021 March 31.
PMID: 33821249 PMCID: PMC8011639 Free PMC article