nicotinamide riboside kinase 2; NRK 2; NmR-K 2; integrin beta-1-binding protein 3; muscle integrin-binding protein; MIBP; nicotinic acid riboside kinase 2; ribosylnicotinamide kinase 2; RNK 2; ribosylnicotinic acid kinase 2 (ITGB1BP3, NRK2, NMRK2)

Function: 1) cofactor biosynthesis; NAD+ biosynthesis 2) attenuates laminin matrix deposition & cell adhesion to laminin, but not to fibronectin 3) regulation of PXN & PXN tyrosine phosphorylation 4) regulation of terminal myogenesis 5) catalyzes synthesis of nicotinamide nucleotide from nicotinamide riboside 6) Preiss-Handler independent NAD biosynthesis. 7) interacts with ITGB1 ATP + N-ribosylnicotinamide = ADP + nicotinamide ribonucleotide Kinetic parameters: - KM=0.19 mM for nicotinamide riboside (with ATP as cosubstrate) - KM=30 mM for nicotinamide riboside (with GTP as cosubstrate) - KM=0.11 mM for tiazofurin (with ATP as cosubstrate) - KM=0.063 mM for nicotinic acid riboside (with ATP as cosubstrate) - KM=1.3 mM for uridine (with ATP as cosubstrate) Structure: - monomer (putative) - belongs to the uridine kinase family, NRK subfamily Alternative splicing: named isoforms=2 Expression: - expressed predominantly in skeletal muscle - expressed at a lower level in heart (at protein level) - not expressed in brain, kidney, liver, lung, pancreas or placenta - down-regulated during myoblast differentiation

Properties

SIZE: entity length = 230 aa MW = 26 kD MOTIF: ATP-binding site NAME: ATP-binding site SITE: 9-17 MOTIF: Mg+2-binding site SITE: 16-16 aspartate residue {D35} Mg+2-binding site SITE: 35-35 binding site SITE: 55-55 FOR-BINDING-OF: Substrate kinase domain MOTIF: ATP-binding site NAME: ATP-binding site ATP-binding site NAME: ATP-binding site SITE: 130-130 binding site SITE: 131-131 FOR-BINDING-OF: Substrate ATP-binding site NAME: ATP-binding site SITE: 134-134

Database Correlations

OMIM 608705 UniProt Q9NPI5 Entrez Gene 27231 Kegg hsa:27231 ENZYME correlations

References

UniProt :accession Q9NPI5