nicotinamide riboside kinase 1; NRK 1; NmR-K 1; nicotinic acid riboside kinase 1; ribosylnicotinamide kinase 1; RNK 1; ribosylnicotinic acid kinase 1 (NRK1, NMRK1, C9orf95)

Function: - catalyzes phosphorylation of nicotinamide riboside & nicotinic acid riboside to form nicotinamide mononucleotide & nicotinic acid mononucleotide - also phosphorylates the antitumor drugs tiazofurin & 3-deazaguanosine - cofactor biosynthesis; NAD⁺ biosynthesis ATP + N-ribosylnicotinamide = ADP + nicotinamide ribonucleotide Kinetic parameters: - KM=0.088 mM for nicotinamide riboside (ATP as cosubstrate) - KM=0.068 mM for nicotinamide riboside (GTP as cosubstrate) - KM=0.27 mM for tiazofurin (ATP as cosubstrate) - KM=0.051 mM for nicotinic acid riboside (ATP as cosubstrate) - KM=17 mM for uridine (ATP as cosubstrate) - pH dependence: optimum pH is 6.5-9 Structure: - monomer - belongs to the uridine kinase family, NRK subfamily Alternative splicing: named isoforms=2

Properties

SIZE: entity length = 199 aa MW = 23 kD MOTIF: ATP-binding site NAME: ATP-binding site SITE: 10-18 MOTIF: Mg+2-binding site SITE: 17-17 aspartate residue {D36} Mg+2-binding site SITE: 36-36 binding site SITE: 56-56 FOR-BINDING-OF: Substrate kinase domain MOTIF: ATP-binding site NAME: ATP-binding site ATP-binding site NAME: ATP-binding site SITE: 128-128 binding site SITE: 129-129 FOR-BINDING-OF: Substrate ATP-binding site NAME: ATP-binding site SITE: 132-132 ATP-binding site NAME: ATP-binding site SITE: 172-174

Database Correlations

OMIM 608704 UniProt Q9NWW6 Entrez Gene 54981 Kegg hsa:54981 ENZYME correlations

References

UniProt :accession Q9NWW6