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neurofilament medium polypeptide; NF-M; neurofilament triplet M protein; 160 kD neurofilament protein; neurofilament 3 (NEFM NEF3 NFM)
Function:
- neurofilament protein
- can form neurofilaments in association with neurofilament L [3]
- role in maintenance of neuronal caliber
- phosphorylated on a number of the Ser in K-S-P repeats
- phosphorylation results in the formation of interfilament cross bridges that are important in the maintenance of axonal caliber
- phosphorylation seems to play a major role in functioning of the larger neurofilament polypeptides (NF-M & NF-H); levels of phosphorylation are altered developmentally & coincide with a change in the neurofilament function
Structure:
- repeats containing tripeptide K-S-P
- belongs to the intermediate filament family
Laboratory:
- neurofilament medium chain Ab in CSF
Interactions
molecular events
General
neurofilament protein
Properties
SIZE: entity length = 916 aa
MW = 102 kD
COMPARTMENT: cytoplasm
CELL: neuron
MOTIF: Head {2-104}
acetylation site
SITE: N-TERMINUS
EFFECTOR-BOUND: acetyl
Thr glycosylation site {T47}
Rod {105-412}
MOTIF: Coil 1A {105-136}
Linker 1 {137-149}
Coil 1B {150-248}
Linker 12 {249-265}
Coil 2A {266-287}
Linker 2 {288-291}
Coil 2B {292-412}
Tyr phosphorylation site {Y320}
Tail {413-916}
MOTIF: Thr glycosylation site {T431}
Ser phosphorylation site {S511}
Ser phosphorylation site {S545}
Ser phosphorylation site {S553}
Ser phosphorylation site {S559}
consensus repeat {614-691} (6)
Ser phosphorylation site {S680}
Ser phosphorylation site {S685}
Ser phosphorylation site {S736}
Ser phosphorylation site {S783}
Ser phosphorylation site {S788}
Ser phosphorylation site {S837}
Database Correlations
OMIM 162250
UniProt P07197
PFAM correlations
References
- Alperts et al Molecular Biology of the Cell, Garland
Publishing, New York, NY 1989 pg 662
- Wang KK et al
Calmodulin-binding proteins as calpain substrates.
Biochem J 262:693 1989
PMID: 2556106
- Cairns NJ, Lee VM, Trojanowski JQ.
The cytoskeleton in neurodegenerative diseases.
J Pathol. 2004 Nov;204(4):438-49. Review.
PMID: 15495240
- UniProt :accession P07197
- Human intermediate Filament mutation Database
http://www.interfil.org