nBAF complex (neuron-specific chromatin remodeling complex)

Function: - chromatin remodeling - during neural development a switch from a stem/progenitor to a post-mitotic chromatin remodeling mechanism occurs as neurons exit the cell cycle & become committed to their adult state - the transition from proliferating neural stem/progenitor cells to post-mitotic neurons requires a switch in subunit composition of the npBAF & nBAF complexes - as neural progenitors exit mitosis & differentiate into neurons, npBAF complexes which contain ACTL6A/BAF53A & PHF10/BAF45A, are exchanged for homologous alternative ACTL6B/BAF53B & DPF1/BAF45B or DPF3/BAF45C subunits in nBAF complexes - the nBAF complex along with CREST plays a role regulating the activity of genes essential for dendrite growth Structure: - subunits: ARID1A/BAF250A or ARID1B/BAF250B, SMARCD1/BAF60A, SMARCD3/BAF60C, SMARCA2/BRM/BAF190B, SMARCA4/BRG1/BAF190A, SMARCB1/BAF47, SMARCC1/BAF155, SMARCE1/BAF57, SMARCC2/BAF170, DPF1/BAF45B, DPF3/BAF45C, ACTL6B/BAF53B & actin

Properties

COMPARTMENT: cell nucleus SUBUNITS: AT-rich interactive domain-containing protein 1A COMPARTMENT: cell nucleus MOTIF: acetylation site SITE: N-TERMINUS EFFECTOR-BOUND: acetyl Ser phosphorylation site {S79} Ser phosphorylation site {S233} Thr phosphorylation site {T286} LXXLL {295-299} Ser phosphorylation site {S363} Ser phosphorylation site {S382} glutamine-rich region {479-482} MOTIF: glutamine residue (SEVERAL) glutamine-rich region {561-567} MOTIF: glutamine residue (SEVERAL) Ser phosphorylation site {S604} Ser phosphorylation site {S696} Ser phosphorylation site {S698} Ser phosphorylation site {S702} Ser phosphorylation site {S764} Ser phosphorylation site {S772} serine-rich region {998-1001} MOTIF: serine residue (SEVERAL) ARID {1017-1108} Ser phosphorylation site {S1184} glutamine-rich region {1327-1404} MOTIF: glutamine residue (SEVERAL) LXXLL {1709-1713} Ser phosphorylation site {S1755} Ser phosphorylation site {S1944} LXXLL {1967-1971} LXXLL {2085-2089} AT-rich interactive domain-containing protein 1B COMPARTMENT: NUCL :NAME ARID MOTIF: DNA-binding motif SWI/SNF-related matrix-associated actin-dependent regulator of chromatin subfamily D member 1 COMPARTMENT: cell nucleus MOTIF: multiple interactions {43-167} MOTIF: lysine-rich region {124-127} MOTIF: lysine residue (SEVERAL) SMARCC1/SMARCC2 interaction {168-474} domain {180-515} MOTIF: SWIB {291-390} coiled coil {412-440} SWI/SNF-related matrix-associated actin-dependent regulator of chromatin subfamily D member 3 COMPARTMENT: cell nucleus MOTIF: Ser phosphorylation site {S178} SWIB {259-334} global transcription activator SNF2L2 COMPARTMENT: cell nucleus MOTIF: Ser phosphorylation site {S175} glutamine-rich region {216-238} MOTIF: glutamine residue (SEVERAL) glutamine-rich region {245-253} MOTIF: glutamine residue (SEVERAL) Ser phosphorylation site {S329} HSA {436-508} arginine-rich region {559-562} MOTIF: arginine residue (SEVERAL) Ser phosphorylation site {S591} glutamate-rich region {643-650} MOTIF: glutamate residue (SEVERAL) helicase NAME: helicase SITE: 736-901 MOTIF: ATP-binding site NAME: ATP-binding site SITE: 749-756 DEAD/H box NAME: DEAD/H box SITE: 851-854 Ser phosphorylation site {S956} Helicase C-terminal {1054-1216} glutamate-rich region {1297-1301} MOTIF: glutamate residue (SEVERAL) Ser phosphorylation site {S1377} Ser phosphorylation site {S1410} Ser phosphorylation site {S1418} bromodomain {1419-1489} MOTIF: Ser phosphorylation site {S1419} Ser phosphorylation site {S1512} Ser phosphorylation site {S1516} glutamate-rich region {1518-1529} MOTIF: glutamate residue (SEVERAL) Ser phosphorylation site {S1528} Ser phosphorylation site {S1568} Ser phosphorylation site {S1572} Ser phosphorylation site {S1582} Ser phosphorylation site {S1585} Thr phosphorylation site {T1587} transcription activator BRG1 COMPARTMENT: cell nucleus MOTIF: SS18L1/CREST interaction {1-282} MOTIF: Thr phosphorylation site {T11} HSA {460-532} lysine-rich region {578-588} MOTIF: lysine residue (SEVERAL) Thr phosphorylation site {T609} Ser phosphorylation site {S610} Ser phosphorylation site {S613} Ser phosphorylation site {S655} Ser phosphorylation site {S657} Ser phosphorylation site {S660} Ser phosphorylation site {S662} glutamate-rich region {663-672} MOTIF: glutamate residue (SEVERAL) Ser phosphorylation site {S695} Ser phosphorylation site {S699} Ser phosphorylation site {S721} helicase NAME: helicase SITE: 766-931 MOTIF: ATP-binding site NAME: ATP-binding site SITE: 779-786 DEAD/H box NAME: DEAD/H box SITE: 881-884 Helicase C-terminal {1084-1246} glutamate-rich region {1360-1364} MOTIF: glutamate residue (SEVERAL) Ser phosphorylation site {S1382} Ser phosphorylation site {S1452} bromodomain {1477-1547} MOTIF: peptide motif {1539-1540} Ser phosphorylation site {S1570} glutamate-rich region {1571-1584} MOTIF: glutamate residue (SEVERAL) Ser phosphorylation site {S1575} Ser phosphorylation site {S1586} Ser phosphorylation site {S1627} Ser phosphorylation site {S1631} Ser phosphorylation site {S1640} Ser phosphorylation site {S1642} Ser phosphorylation site {S1644} SWI/SNF-related matrix-associated actin-dependent regulator of chromatin subfamily B member 1 COMPARTMENT: cell nucleus MOTIF: binding site SITE: 106-183 FOR-BINDING-OF: deoxyribonucleic acid (DNA) MOTIF: Ser phosphorylation site {S129} binding site SITE: 183-243 FOR-BINDING-OF: HIV-1 INTEGRASE tandem repeats {186-319} MOTIF: consensus repeat {186-245} binding site SITE: 186-245 FOR-BINDING-OF: myc protein family consensus repeat {259-319} PPP1R15A interaction {304-318} SWI/SNF complex subunit SMARCC1 COMPARTMENT: cell nucleus MOTIF: acetylation site SITE: N-TERMINUS EFFECTOR-BOUND: acetyl Ser phosphorylation site {S301} Ser phosphorylation site {S310} Ser phosphorylation site {S319} Ser phosphorylation site {S328} proline-rich region SITE: 329-336 MOTIF: proline residue (SEVERAL) Ser phosphorylation site {S330} Thr phosphorylation site {T335} Thr phosphorylation site {T337} Ser phosphorylation site {S339} Ser phosphorylation site {S357} SWIRM {449-546} Ser phosphorylation site {S573} SANT {618-669} glutamate-rich region {769-863} MOTIF: glutamate residue (SEVERAL) alanine-rich region {867-878} MOTIF: alanine residue (SEVERAL) coiled coil {914-946} proline-rich region SITE: 977-1105 MOTIF: proline residue (SEVERAL) SWI/SNF-related matrix-associated actin-dependent regulator of chromatin subfamily E member 1 COMPARTMENT: cell nucleus MOTIF: proline-rich region SITE: 5-65 MOTIF: proline residue (SEVERAL) HMG box SITE: 66-134 coiled coil {220-319} MOTIF: Ser phosphorylation site {S265} glutamate-rich region {320-411} MOTIF: glutamate residue (SEVERAL) SWI/SNF complex subunit SMARCC2 COMPARTMENT: cell nucleus MOTIF: glutamate-rich region {186-189} MOTIF: glutamate residue (SEVERAL) Ser phosphorylation site {S283} Ser phosphorylation site {S286} Tyr phosphorylation site {Y296} Ser phosphorylation site {S302} Ser phosphorylation site {S304} Ser phosphorylation site {S306} Thr phosphorylation site {T322} Ser phosphorylation site {S347} SWIRM {424-521} SANT {596-647} glutamate-rich region {747-855} MOTIF: glutamate residue (SEVERAL) Ser phosphorylation site {S806} Ser phosphorylation site {S810} alanine-rich region {861-870} MOTIF: alanine residue (SEVERAL) coiled coil {907-934} glutamine-rich region {956-960} MOTIF: glutamine residue (SEVERAL) proline-rich region SITE: 961-1213 MOTIF: proline residue (SEVERAL) zinc finger protein neuro-d4 COMPARTMENT: cytoplasm cell nucleus MOTIF: Zn finger PHD-type NAME: Zn finger PHD-type SITE: 254-311 EFFECTOR-BOUND: Zn+2 Zn finger PHD-type NAME: Zn finger PHD-type SITE: 308-368 EFFECTOR-BOUND: Zn+2 zinc-finger protein DPF3 COMPARTMENT: cytoplasm cell nucleus MOTIF: glycine-rich region {200-203} actin-like protein 6B COMPARTMENT: cell nucleus MOTIF: domain {39-82} actin MOTIF: coiled coil

References

UnitProt :accession P51531

Components

actin actin-like protein 6B; 53 kD BRG1-associated factor B; actin-related protein Baf53b; ArpNalpha; BRG1-associated factor 53B; BAF53B (ACTL6B, ACTL6, BAF53B) AT-rich interactive domain-containing protein 1A; ARID domain-containing protein 1A; SWI/SNF-related, matrix-associated, actin-dependent regulator of chromatin subfamily F member 1; SWI/SNF complex protein p270; B120; SWI-like protein; Osa homolog 1; hOSA1; hELD; BRG1-associated factor 250; BAF250; BRG1-associated factor 250a; BAF250A (ARID1A, BAF250, BAF250A, C1orf4, OSA1, SMARCF1) AT-rich interactive domain-containing protein 1B (ARID domain-containing protein 1B, Osa homolog 2, hOsa2, p250R, BRG1-binding protein hELD/OSA1, BRG1-associated factor 250b, BAF250B, ARID1B, DAN15, KIAA1235, OSA2) SWI/SNF-related matrix-associated actin-dependent regulator of chromatin subfamily A member 2; global transcription activator SNF2L2; ATP-dependent helicase SMARCA2; SNF2-alpha; protein brahma homolog; hBRM; BRG1-associated factor 190B; BAF190B (SMARCA2, BAF190B, BRM, SNF2A, SNF2L2) SWI/SNF-related matrix-associated actin-dependent regulator of chromatin subfamily A member 4; transcription activator BRG1; ATP-dependent helicase SMARCA4; SNF2-beta; protein BRG-1; mitotic growth & transcription activator; protein brahma homolog 1; BRG1-associated factor 190A; BAF190A (SMARCA4, BAF190A, BRG1, SNF2B, SNF2L4) SWI/SNF-related matrix-associated actin-dependent regulator of chromatin subfamily B member 1; integrase interactor 1 protein; SNF5 homolog; hSNF5; BRG1-associated factor 47; BAF47 (SMARCB1, BAF47, INI1, SNF5L1) SWI/SNF-related matrix-associated actin-dependent regulator of chromatin subfamily C member 1; SWI/SNF complex subunit SMARCC1; SWI/SNF complex 155 kD subunit; BRG1-associated factor 155; BAF155 (SMARCC1, BAF155) SWI/SNF-related matrix-associated actin-dependent regulator of chromatin subfamily C member 2; SWI/SNF complex subunit SMARCC2; SWI/SNF complex 170 kD subunit; BRG1-associated factor 170; BAF170 (SMARCC2, BAF170) SWI/SNF-related matrix-associated actin-dependent regulator of chromatin subfamily D member 1; SWI/SNF complex 60 kD subunit; 60 kD BRG-1/Brm-associated factor subunit A; BRG1-associated factor 60A; BAF60A (SMARCD1, BAF60A) SWI/SNF-related matrix-associated actin-dependent regulator of chromatin subfamily D member 3; 60 kD BRG-1/Brm-associated factor subunit C; BRG1-associated factor 60C; BAF60C (SMARCD3, BAF60C) SWI/SNF-related matrix-associated actin-dependent regulator of chromatin subfamily E member 1; BRG1-associated factor 57; BAF57 (SMARCE1, BAF57) zinc finger protein neuro-d4; BRG1-associated factor 45B; BAF45B; D4, Zn+2 & double PHD fingers family 1 (DPF1, BAF45B, NEUD4) zinc-finger protein DPF3 (cer-d4, DPF3, CERD4)