NADPH-dependent diflavin oxidoreductase 1 (novel reductase 1, NADPH-dependent FMN & FAD containing oxidoreductase, NDOR1, NR1)

Function: - oxidoreductase that catalyzes the NADP-dependent reduction of cytochrome c & one-electron acceptors, such as doxorubicin, K⁺ ferricyanide & menadione (in vitro) - interacts with DCPS Cofactor: FMN, FAD Kinetic parameters: - KM=21 uM for cytochrome c - Vmax=1.2 umol/min/ug enzyme for cytochrome c reduction - Optimum pH is about 8.0 Structure: - in the C-terminal section, belongs to the flavoprotein pyridine nucleotide cytochrome reductase family - contains 1 FAD-binding domain - contains 1 flavodoxin-like domain Compartment: cytoplasm, concentrated in perinuclear structure Alternative splicing: named isoforms=2 Expression: - low expression in brain, heart, kidney, pancreas, prostate & skeletal muscle - highest levels in the placenta Pathology: - expressed in cancer cell lines including promyelocytic leukemia, HeLa S3, chronic myelogenous leukemia, lymphoblastic leukemia, Burkitt's lymphoma, colorectal adenocarcinoma, lung carcinoma, & melanoma G361 - overexpression enhances cytotoxicity of menadione, & consequently induced cell death; coexpression with DCPS diminished this toxicity

Properties

SIZE: entity length = 597 aa MW = 67 kD COMPARTMENT: cytoplasm cell nucleus MOTIF: Flavodoxin-like {6-150} proline-rich region SITE: 158-161 MOTIF: proline residue (SEVERAL) binding site SITE: 201-421 FOR-BINDING-OF: flavin adenine dinucleotide MOTIF: cofactor-binding site [242-253] COFACTOR-BOUND: flavin adenine dinucleotide cofactor-binding site [379-389] COFACTOR-BOUND: flavin adenine dinucleotide cofactor-binding site [454-472] COFACTOR-BOUND: NADP cofactor-binding site [543-559] COFACTOR-BOUND: NADP

Database Correlations

OMIM 606073 UniProt Q9UHB4 PFAM correlations Kegg hsa:2715

References

UniProt :accession Q9UHB4