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myelin regulatory factor; myelin gene regulatory factor; contains: myelin regulatory factor, N-terminal; myelin regulatory factor, C-terminal (MYRF, C11orf9, KIAA0954, MRF)
Function:
- myelin regulatory factor:
- precursor of the transcription factor
- mediates autocatalytic cleavage that releases the myelin regulatory factor, N-terminal component that specifically activates transcription of central nervous system (CNS) myelin genes [2]
- myelin regulatory factor, C-terminal:
- membrane-bound part
- has no transcription factor activity
- remains attached to the endoplasmic reticulum membrane following cleavage [2]
- myelin regulatory factor, N-terminal:
- transcription factor
- specifically activates expression of myelin genes such as MBP, MOG, MAG & PLP1 during oligodendrocyte (OL) maturation,
- role in oligodendrocyte maturation & CNS myelination
- specifically recognizes & binds DNA sequence 5'-CTGGYAC-3' in the regulatory regions of myelin-specific genes & directly activates their expression
- not only required during oligodendrocyte differentiation but is also required on an ongoing basis for the maintenance of expression of myelin genes & for the maintenance of a mature, viable oligodendrocyte phenotype [2]
Structure:
- homotrimer
- myelin regulatory factor, N-terminal:
- the nuclear localization signals mediate translocation to the nucleus [2]
- myelin regulatory factor:
- the peptidase S74 domain {intramolecular chaperone auto- processed (ICA) domain or intramolecuar chaperone domain (ICD)} has protease activity & mediates autocatalytic processing of the protein to generate
- the myelin regulatory factor,
- N-terminal active transcription factor
- myelin regulatory factor, C-terminal components [2]
- belongs to the MRF family
- contains 1 NDT80 DNA-binding domain
- contains 1 peptidase S74 domain
Compartment:
- myelin regulatory factor:
- endoplasmic reticulum membrane; single-pass membrane protein
- myelin regulatory factor, N-terminal:
- nucleus, cytoplasm
- translocates from cytoplasm to nucleus upon autocatalytic cleavage
- myelin regulatory factor, C-terminal:
- endoplasmic reticulum membrane; single-pass membrane protein
Alternative splicing: named isoforms=2
Expression:
- expressed in lung, ARPE-19 cell line, brainstem, uterus &, to a lesser extent, in basal ganglion & liver
- weakly expressed in cerebellum & retina
General
DNA-binding protein
glycoprotein
membrane protein
oligomerizing protein
Properties
SIZE: entity length = 1151 aa
MW = 124 kD
COMPARTMENT: cytoplasm
cell nucleus
endoplasmic reticulum
MOTIF: proline-rich region
SITE: 77-330
MOTIF: proline residue (SEVERAL)
DNA-binding motif
SITE: 250-541
MOTIF: nuclear translocation signal {254-257}
nuclear translocation signal {491-494}
proteolytic site {586-587}
Peptidase S74 {587-696}
coiled coil {680-711}
transmembrane domain {769-789}
N-glycosylation site {N919}
N-glycosylation site {N1043}
N-glycosylation site {N1065}
N-glycosylation site {N1129}
Database Correlations
OMIM 608329
UniProt Q9Y2G1
PFAM correlations
Entrez Gene 745
Kegg hsa:745
References
- UniProt :accession Q9Y2G1
- Li Z, Park Y, Marcotte EM.
A Bacteriophage tailspike domain promotes self-cleavage of a
human membrane-bound transcription factor, the myelin regulatory
factor MYRF.
PLoS Biol. 2013;11(8):e1001624
PMID: 23966832