multiple epidermal growth factor-like domains 10 (multiple EGF-like-domains 10, MEGF10, KIAA1780)

Function: - role in phagocytosis by macrophages of apoptotic cells - cooperates with ABCA1 during engulfment - destabilizes the oligomeric assemblies of the ABCA1 transporter - role in cell adhesion - inhibits cell motility & cell proliferation in vitro - promotes formation of large intracellular vacuoles - homopolymer (probable) - component of a complex composed of at least ACTB, AP2M1, AP2A1, AP2A2, & VIM - interacts directly with GULP1 & ABCA1 - interacts with AP2M1 - does not interact with MEGF11 - phosphorylated on Tyr - ubiquinated, mono- & polyubiquitinated forms are detected Structure: - belongs to the MEGF family - contains 15 EGF-like domains - contains 1 EMI domain - EMI & EGF-like domains work in concert to promote self-assembly Compartment: - cell membrane, basolateral cell membrane - enriched at the sites of contact with apoptotic thymocytes (putative) - forms an irregular, mosaic-like adhesion pattern in region of the cell surface that becomes firmely fixed to substrate - expressed at the cell surface in clusters around cell corpses during engulfment - during the engulfment of apoptotic thymocytes, recruited at the bottom of the forming phagocytic cup - colocalizes with ABCA1 in absence of any phagocytic challenge - oes not localize within lamellipodia - does not localize with MEGF11 Alternative splicing: named isoforms=2

General

glycoprotein membrane protein

Properties

SIZE: entity length = 1140 aa MW = 122 kD COMPARTMENT: cellular membrane MOTIF: AP2M1 interaction {1-857} MOTIF: signal sequence {1-25} EMI {30-107} cysteine residue {C34} MODIFICATION: cysteine residue {C95} cysteine residue {C60} MODIFICATION: cysteine residue {C69} cysteine residue {C69} MODIFICATION: cysteine residue {C60} cysteine residue {C94} MODIFICATION: cysteine residue {C105} cysteine residue {C95} MODIFICATION: cysteine residue {C34} cysteine residue {C105} MODIFICATION: cysteine residue {C94} EGF domain {106-136} cysteine residue {C109} MODIFICATION: cysteine residue {C118} cysteine residue {C113} MODIFICATION: cysteine residue {C124} cysteine residue {C118} MODIFICATION: cysteine residue {C109} cysteine residue {C124} MODIFICATION: cysteine residue {C113} cysteine residue {C126} MODIFICATION: cysteine residue {C135} N-glycosylation site {N134} cysteine residue {C135} MODIFICATION: cysteine residue {C126} EGF domain {144-179} cysteine residue {C148} MODIFICATION: cysteine residue {C160} cysteine residue {C154} MODIFICATION: cysteine residue {C167} cysteine residue {C160} MODIFICATION: cysteine residue {C148} cysteine residue {C167} MODIFICATION: cysteine residue {C154} cysteine residue {C169} MODIFICATION: cysteine residue {C178} cysteine residue {C178} MODIFICATION: cysteine residue {C169} EGF domain {187-222} cysteine residue {C191} MODIFICATION: cysteine residue {C203} cysteine residue {C197} MODIFICATION: cysteine residue {C210} cysteine residue {C203} MODIFICATION: cysteine residue {C191} cysteine residue {C210} MODIFICATION: cysteine residue {C197} cysteine residue {C212} MODIFICATION: cysteine residue {C221} cysteine residue {C221} MODIFICATION: cysteine residue {C212} EGF domain {230-265} cysteine residue {C234} MODIFICATION: cysteine residue {C246} cysteine residue {C240} MODIFICATION: cysteine residue {C253} cysteine residue {C246} MODIFICATION: cysteine residue {C234} cysteine residue {C253} MODIFICATION: cysteine residue {C240} cysteine residue {C255} MODIFICATION: cysteine residue {C264} cysteine residue {C264} MODIFICATION: cysteine residue {C255} EGF domain {278-308} cysteine residue {C281} MODIFICATION: cysteine residue {C289} cysteine residue {C283} MODIFICATION: cysteine residue {C296} cysteine residue {C289} MODIFICATION: cysteine residue {C281} cysteine residue {C296} MODIFICATION: cysteine residue {C283} cysteine residue {C298} MODIFICATION: cysteine residue {C307} cysteine residue {C307} MODIFICATION: cysteine residue {C298} EGF domain {316-351} cysteine residue {C320} MODIFICATION: cysteine residue {C332} cysteine residue {C326} MODIFICATION: cysteine residue {C339} cysteine residue {C332} MODIFICATION: cysteine residue {C320} cysteine residue {C339} MODIFICATION: cysteine residue {C326} cysteine residue {C341} MODIFICATION: cysteine residue {C350} cysteine residue {C350} MODIFICATION: cysteine residue {C341} EGF domain {405-440} cysteine residue {C409} MODIFICATION: cysteine residue {C421} cysteine residue {C415} MODIFICATION: cysteine residue {C428} cysteine residue {C421} MODIFICATION: cysteine residue {C409} cysteine residue {C428} MODIFICATION: cysteine residue {C415} cysteine residue {C430} MODIFICATION: cysteine residue {C439} cysteine residue {C439} MODIFICATION: cysteine residue {C430} EGF domain {453-483} cysteine residue {C456} MODIFICATION: cysteine residue {C464} cysteine residue {C458} MODIFICATION: cysteine residue {C471} cysteine residue {C464} MODIFICATION: cysteine residue {C456} cysteine residue {C471} MODIFICATION: cysteine residue {C458} cysteine residue {C473} MODIFICATION: cysteine residue {C482} cysteine residue {C482} MODIFICATION: cysteine residue {C473} EGF domain {491-526} cysteine residue {C495} MODIFICATION: cysteine residue {C507} N-glycosylation site {N496} cysteine residue {C501} MODIFICATION: cysteine residue {C514} cysteine residue {C507} MODIFICATION: cysteine residue {C495} cysteine residue {C514} MODIFICATION: cysteine residue {C501} cysteine residue {C516} MODIFICATION: cysteine residue {C525} cysteine residue {C525} MODIFICATION: cysteine residue {C516} EGF domain {539-569} cysteine residue {C542} MODIFICATION: cysteine residue {C550} cysteine residue {C544} MODIFICATION: cysteine residue {C557} cysteine residue {C550} MODIFICATION: cysteine residue {C542} cysteine residue {C557} MODIFICATION: cysteine residue {C544} cysteine residue {C559} MODIFICATION: cysteine residue {C568} cysteine residue {C568} MODIFICATION: cysteine residue {C559} EGF domain {577-612} cysteine residue {C581} MODIFICATION: cysteine residue {C593} cysteine residue {C587} MODIFICATION: cysteine residue {C600} cysteine residue {C593} MODIFICATION: cysteine residue {C581} cysteine residue {C600} MODIFICATION: cysteine residue {C587} cysteine residue {C602} MODIFICATION: cysteine residue {C611} cysteine residue {C611} MODIFICATION: cysteine residue {C602} EGF domain {665-700} cysteine residue {C669} MODIFICATION: cysteine residue {C681} cysteine residue {C675} MODIFICATION: cysteine residue {C688} cysteine residue {C681} MODIFICATION: cysteine residue {C669} cysteine residue {C688} MODIFICATION: cysteine residue {C675} cysteine residue {C690} MODIFICATION: cysteine residue {C699} cysteine residue {C699} MODIFICATION: cysteine residue {C690} EGF domain {713-743} cysteine residue {C716} MODIFICATION: cysteine residue {C724} cysteine residue {C718} MODIFICATION: cysteine residue {C731} cysteine residue {C724} MODIFICATION: cysteine residue {C716} cysteine residue {C731} MODIFICATION: cysteine residue {C718} cysteine residue {C733} MODIFICATION: cysteine residue {C742} cysteine residue {C742} MODIFICATION: cysteine residue {C733} EGF domain {751-786} cysteine residue {C755} MODIFICATION: cysteine residue {C767} cysteine residue {C761} MODIFICATION: cysteine residue {C774} cysteine residue {C767} MODIFICATION: cysteine residue {C755} cysteine residue {C774} MODIFICATION: cysteine residue {C761} cysteine residue {C776} MODIFICATION: cysteine residue {C785} cysteine residue {C785} MODIFICATION: cysteine residue {C776} EGF domain {799-829} cysteine residue {C802} MODIFICATION: cysteine residue {C810} cysteine residue {C804} MODIFICATION: cysteine residue {C817} cysteine residue {C810} MODIFICATION: cysteine residue {C802} cysteine residue {C817} MODIFICATION: cysteine residue {C804} cysteine residue {C819} MODIFICATION: cysteine residue {C828} cysteine residue {C828} MODIFICATION: cysteine residue {C819} transmembrane domain {858-878} vacuole formation {945-1140} MOTIF: serine-rich region {1119-1139} MOTIF: serine residue (SEVERAL)

Database Correlations

UniProt Q96KG7 PFAM correlations

References

UniProt :accession Q96KG7