mRNA cap guanine-N7 methyltransferase; RG7MT1; mRNA (guanine-N(7)-)-methyltransferase; mRNA cap methyltransferase; hCMT1; hMet; hcm1p (RNMT KIAA0398)

Function: 1) mRNA capping methyltransferase 2) methylates N7 position of the added guanosine to the 5' cap structure of mRNAs 3) binds RNA containing 5'-terminal GpppC 4) interacts with importin alpha - stimulates RNA-binding & methyltransferase activity 5) interaction with importin beta 6) interacts with elongating form of polymerase 2 & RNGTT S-adenosyl-L-methionine + G(5')pppR-RNA S-adenosyl-L-homocysteine + m(7)G(5')pppR-RNA Structure: - belongs to the methyltransferase superfamily, mRNA cap methyltransferase family Compartment: nucleus Alternative splicing: named isoforms=2 Expression: widely expressed

General

nuclear protein N-methyltransferase phosphoprotein

Properties

SIZE: entity length = 476 aa MW = 55 kD COMPARTMENT: cell nucleus MOTIF: Ser phosphorylation site {S24} Ser phosphorylation site {S28} nuclear translocation signal {126-128} mRNA cap binding {176-177} binding site SITE: 180-180 FOR-BINDING-OF: S-adenosylmethionine binding site SITE: 205-205 FOR-BINDING-OF: S-adenosylmethionine binding site SITE: 208-208 FOR-BINDING-OF: mRNA cap binding site SITE: 214-214 FOR-BINDING-OF: mRNA cap binding site SITE: 227-227 FOR-BINDING-OF: S-adenosylmethionine binding site SITE: 239-239 FOR-BINDING-OF: mRNA cap (Probable) binding site SITE: 261-261 FOR-BINDING-OF: S-adenosylmethionine binding site SITE: 284-284 FOR-BINDING-OF: S-adenosylmethionine binding site SITE: 288-288 FOR-BINDING-OF: mRNA cap binding site SITE: 370-370 FOR-BINDING-OF: mRNA cap binding site SITE: 467-467 FOR-BINDING-OF: mRNA cap

Database Correlations

OMIM 603514 UniProt O43148 Pfam PF03291 Entrez Gene 8731 Kegg hsa:8731 ENZYME 2.1.1.56

References

UniProt :accession O43148