mitotic spindle assembly checkpoint protein MAD1 (mitotic arrest deficient-like protein 1, MAD1-like 1, mitotic checkpoint MAD1 protein-homolog, HsMAD1, hMAD1, Tax-binding protein 181, MAD1L1, MAD1, TXBP181)

Function: 1) component of the spindle-assembly checkpoint 2) role in the correct positioning of the septum 3) anchoring MAD2L1 to the nuclear periphery 4) forms tetrameric MAD1L1-MAD2L1 complex 5) CDC20 can compete with MAD1L1 for MAD2L1 binding, until attachment &/or tension dampen the checkpoint signal 6) able to interact with the BUB1/BUB3 complex 7) induced by Tp53 8) phosphorylated by BUB1 - become hyperphosphorylated in late S phase through M phase or after mitotic spindle damage Compartment: - nucleus - moves from diffusely nuclear distribution to the centrosome, to the spindle midzone & finally to the midbody during mitosis Alternative splicing: named isoforms=2 Expression: - expressed weakly at G0/G1 & highly at late S phase & G2/M phase Pathology: 1) interacts with viral Tax protein 2) defects in MAD1L1 are involved in development &/or progression of various types of cancer

General

nuclear protein phosphoprotein

Properties

SIZE: MW = 83 kD entity length = 718 aa COMPARTMENT: cell nucleus MOTIF: coiled coil {46-632} MOTIF: Ser phosphorylation site {S428}

Database Correlations

OMIM 602686 MORBIDMAP 602686 UniProt Q9Y6D9 Pfam PF05557

References

UniProt :accession Q9Y6D9