microtubule-associated protein RP/EB family member 2 (APC-binding protein EB2, end-binding protein 2, EB2, MAPRE2, RP1)

Function: 1) role in microtubule polymerization & spindle function by stabilizing microtubules & anchoring them at centrosomes (putative) 2) role in cell migration (putative) 3) interacts with DCTN1 4) binds to C-terminal domain of APC 5) binds monomeric & polymerized tubulin Structure: 1) composed of 2 functionally independent domains - N-terminal domain forms an hydrophobic cleft involved in microtubule binding - C-terminal domain is involved in formation of mutually exclusive complexes with APC & DCTN1 2) belongs to the MAPRE family 3) contains 1 CH domain (calponin-homology domain) - contains 1 EB1 C-terminal domain Compartment: - cytoplasm - associated with microtubule network - accumulates at plus end of microtubules Alternative splicing: named isoforms=2 Expression: - up-regulated in activated B-cells & T-lymphocytes Pathology: - expressed in different tumor cell lines

General

microtubule-associated protein (MAP) phosphoprotein

Properties

SIZE: MW = 37 kD entity length = 327 aa COMPARTMENT: cytoplasm MOTIF: calponin homology domain NAME: calponin homology domain SITE: 57-159 Tyr phosphorylation site {Y167} binding site SITE: 187-327 FOR-BINDING-OF: dynactin-1 MOTIF: EB1 C-terminal {236-306} binding site SITE: 259-302 FOR-BINDING-OF: adenomatous polyposis coli protein

Database Correlations

OMIM 605789 UniProt Q15555 PFAM correlations

References

UniProt :accession Q15555