microtubule-actin cross-linking factor 1, isoforms 1/2/3/5; actin cross-linking family protein 7; macrophin-1; trabeculin-alpha; 620 kD actin-binding protein; ABP620 (MACF1, ABP620, ACF7, KIAA0465, KIAA1251)

Function: - F-actin-binding protein - may play a role in cross-linking actin to other cytoskeletal proteins - also binds to microtubules (putative) Structure: - belongs to the plakin or cytolinker family - contains 1 actin-binding domain - contains 2 CH (calponin-homology) domains - contains 2 EF-hand domains - contains 1 GAR domain - contains 1 SH3 domain - contains 37 spectrin repeats Compartment: cytoplasm, cytoskeleton Alternative splicing: named isoforms=5 Expression: ubiquitously expressed

Properties

SIZE: entity length = 5430 aa MW = 620 kD COMPARTMENT: cytoplasm MOTIF: actin-binding site SITE: 1-295 FOR-BINDING-OF: F-actin MOTIF: calponin homology domain NAME: calponin homology domain SITE: 78-181 calponin homology domain NAME: calponin homology domain SITE: 194-295 coiled coil {243-265} Ser phosphorylation site {S280} spectrin repeat {314-355} coiled coil {477-529} spectrin repeat {591-623} spectrin repeat {680-784} MOTIF: coiled coil {722-751} spectrin repeat {786-800} coiled coil {816-843} src homology 3 [SH3] domain SITE: 871-923 coiled coil {1013-1118} coiled coil {1164-1191} spectrin repeat {1250-1272} spectrin repeat {1287-1342} Ser phosphorylation site {S1376} coiled coil {1399-1690} MOTIF: spectrin repeat {1455-1534} spectrin repeat {1547-1659} coiled coil {1780-1843} spectrin repeat {1815-1891} MOTIF: Ser phosphorylation site {S1860} spectrin repeat {1932-2042} MOTIF: coiled coil {1975-2005} coiled coil {2039-2312} MOTIF: spectrin repeat {2260-2280} spectrin repeat {2372-2395} coiled coil {2385-2417} spectrin repeat {2398-2507} MOTIF: Ser phosphorylation site {S2454} spectrin repeat {2510-2618} coiled coil {2544-2695} spectrin repeat {2621-2728} spectrin repeat {2731-2838} MOTIF: coiled coil {2760-2838} spectrin repeat {2841-2945} coiled coil {2911-3001} spectrin repeat {2987-3024} coiled coil {3130-3164} MOTIF: spectrin repeat {3136-3163} spectrin repeat {3187-3274} coiled coil {3244-3277} spectrin repeat {3277-3383} spectrin repeat {3386-3492} MOTIF: coiled coil {3418-3482} spectrin repeat {3495-3601} coiled coil {3596-3666} spectrin repeat {3604-3673} spectrin repeat {3713-3819} MOTIF: coiled coil {3786-3806} spectrin repeat {3832-3927} coiled coil {3852-3931} coiled coil {3967-3987} spectrin repeat {3982-4043} spectrin repeat {4046-4152} MOTIF: Ser phosphorylation site {S4074} coiled coil {4084-4218} spectrin repeat {4155-4262} Spectrin {4265-4371} coiled coil {4343-4378} Spectrin {4374-4481} MOTIF: coiled coil {4408-4437} coiled coil {4468-4498} Spectrin {4484-4590} Spectrin {4593-4700} Spectrin {4707-4808} Spectrin {4811-4917} coiled coil {4907-4935} Spectrin {4920-4985} Ser phosphorylation site {S5009} Spectrin {5030-5054} coiled coil {5044-5067} EF hand SITE: 5083-5118 MOTIF: Ca+2-binding site SITE: 5096-5107 EF hand SITE: 5119-5154 MOTIF: Ca+2-binding site SITE: 5132-5143 GAR {5159-5231} serine-rich region {5276-5283} MOTIF: serine residue (SEVERAL) Ser phosphorylation site {S5330} Ser phosphorylation site {S5332} Ser phosphorylation site {S5334} 4 X 4 AA tandem repeats of [GS]-S-R-[AR] {5355-5370} Ser phosphorylation site {S5372} Ser phosphorylation site {S5375}

Database Correlations

OMIM 608271 UniProt Q9UPN3 PFAM correlations Entrez Gene 23499 Kegg hsa:23499

References

UniProt :accession Q9UPN3