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metaphase chromosome protein 1; metaphase chromosome protein 30 (HSMCR30, MCP1, MCP30)

Function: - required during early stages of DNA replication such as the G1/S transition phase & early S phase - phosphorylated - associates with replication factors required for the initiation of DNA replication & binds to the initiation sites in loci that replicate early in S-phase - the 31 kD form interacts with CDC6, ORC2, ORC4, MCM2, MCM3, MCM7, CDC45 & PCNA - both the 31 kD & 33 kD forms interact with HIST1H1A, HIST1H1B & HIST1H1C Compartment: - nucleus, chromosome - associated with condensed chromosomes throughout mitosis - during interphase, shows a speckle distribution in the nucleus, excluding the nucleolus Notes: - 2 forms: 33 kD, & 31 kD are probably produced by initiation at Met-1 & Met-24 respectively - uncertain whether Met-1 or Met-24 is the initiator - product of a dubious CDS prediction - not possible to identify the locus for this potential gene on the reference genome & similarity searches suggest it might not be of human origin evidences presented in refs [2=4] - - UniProt entry deleted

General

nuclear protein

Properties

SIZE: entity length = 290 aa MW = 33 kD COMPARTMENT: cell nucleus

References

  1. UniProt :accession P0CW71
  2. Bronze-da-Rocha E et al Molecular cloning of metaphase chromosome protein 1 (MCP1), a novel human autoantigen that associates with condensed chromosomes during mitosis. Chromosome Res. 1998 Feb;6(2):85-95. PMID: 9543011
  3. Bronze-da-Rocha E et al The human autoantigen MCP1 is required during early stages of DNA replication. Chromosome Res. 2000;8(8):699-711. PMID: 11196133
  4. Bronze-da-Rocha E et al Interactions of MCP1 with components of the replication machinery in mammalian cells. Int J Biol Sci. 2011 Feb 17;7(2):193-208. PMID: 21383955