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membrane copper amine oxidase (semicarbazide-sensitive amine oxidase, SSAO, vascular adhesion protein 1, VAP-1, human placenta amine oxidase, HPAO, AOC3, VAP1)
Function:
1) cell adhesion protein that participates in lymphocyte recirculation by mediating the binding of lymphocytes to peripheral lymph node vascular endothelial cells (L-selectin independent) fashion
2) monoamine oxidase activity
3) topaquinone (TPQ) is generated by Cu+2-dependent autoxidation of a specific tyrosyl residue
RCH2NH2 + H2O + O2 RCHO + NH3 + H2O2
Cofactor:
- binds 1 Cu+2 per subunit
- binds 2 Ca+2 per subunit
- contains 1 topaquinone per subunit
Structure:
1) homodimer, disulfide-linked
2) belongs to the copper/topaquinone oxidase family
3) N- & O-glycosylated
Compartment: membrane
Expression:
- expressed in high endothelial venules of peripheral lymph nodes & on hepatic endothelia
- expressed in appendix, lung, small intestine > bone marrow, colon, heart, kidney, ovary, pancreas, placenta, prostate, skeletal muscle, spleen, testis
General
adhesion receptor
glycoprotein
metalloprotein
oxidoreductase
Properties
SIZE: MW = 84 kD
entity length = 762 aa
COMPARTMENT: plasma membrane
MOTIF: transmembrane domain {5-25}
N-glycosylation site {N136}
cysteine residue {C197}
MODIFICATION: cysteine residue {C198}
cysteine residue {C198}
MODIFICATION: cysteine residue {C197}
Thr glycosylation site {T211}
N-glycosylation site {N231}
N-glycosylation site {N293}
aspartate residue {D385}
tyrosine residue {Y470}
copper [Cu]-binding site
SITE: 519-519
MOTIF: histidine residue (3)
copper [Cu]-binding site
SITE: 521-521
MOTIF: histidine residue (3)
Ca+2-binding site
SITE: 528-528
Ca+2-binding site
SITE: 529-529
Ca+2-binding site
SITE: 530-530
Ca+2-binding site
SITE: 571-571
N-glycosylation site {N591}
N-glycosylation site {N617}
Ca+2-binding site
SITE: 637-637
Ca+2-binding site
SITE: 662-662
Ca+2-binding site
SITE: 664-664
N-glycosylation site {N665}
Ca+2-binding site
SITE: 666-666
Ca+2-binding site
SITE: 672-672
Ca+2-binding site
SITE: 673-673
copper [Cu]-binding site
SITE: 683-683
MOTIF: histidine residue (3)
cysteine residue {C733}
MODIFICATION: cysteine residue {C740}
cysteine residue {C740}
MODIFICATION: cysteine residue {C733}
cysteine residue {C747}
MODIFICATION: cysteine residue {C-INTERCHAIN}
Database Correlations
OMIM 603735
UniProt Q16853
PFAM correlations
ENZYME 1.4.3.6
References
UniProt :accession Q16853