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membrane-associated tyrosine-& threonine-specific cdc2-inhibitory kinase; Myt1 kinase (PKMYT1, MYT1)
Function:
- negative regulator of entry into mitosis (G2to M transition)
a) catalyzes phosphorylation CDC2 kinase complexed to cyclin
b) inhibit mitotic entry by cytoplasmic retention of CDC2-CCNB1 complexes
c) negatively regulated by hyperphosphorylation during mitosis
d) phosphorylated by CDC2-CCNB1 complexes, PLK1 protein kinase?
e) hyperphosphorylated form does not associate with CDC2-CCNB1 complexes
Structure:
- the membrane-association motif is essential for the localization to membrane of Golgi stack
- belongs to the protein kinase superfamily, Ser/Thr protein kinase family, WEE1 subfamily
- contains 1 protein kinase domain
Compartment:
- membrane-associated
- Golgi & endoplasmic reticulum
General
serine/threonine kinase
tyrosine kinase (protein tyrosine kinase, PTK)
Properties
SIZE: MW = 55 kD
entity length = 499 aa
COMPARTMENT: golgi
endoplasmic reticulum
STATE: active state
MOTIF: proline-rich region
SITE: 5-72
MOTIF: proline residue (SEVERAL)
kinase domain
SITE: 110-359
MOTIF: ATP-binding site
NAME: ATP-binding site
SITE: 116-124
ATP-binding site
NAME: ATP-binding site
SITE: 139-139
aspartate residue {D233}
Membrane-association {382-398}
binding site
SITE: 398-499
FOR-BINDING-OF: peptidyl-prolyl cis-trans isomerase NIMA-interacting 1
MOTIF: Ser phosphorylation site {S426}
binding site
SITE: 437-499
FOR-BINDING-OF: cyclin-dependent kinase-1
Thr phosphorylation site {T495}
Database Correlations
OMIM 602474
UniProt Q99640
Pfam PF00069
Entrez Gene 9088
Kegg hsa:9088
ENZYME 2.7.11.1
References
- UniProt :accession Q99640
- NIEHS-SNPs
http://egp.gs.washington.edu/data/pkmyt1/