mediator complex (mediator of RNA polymerase 2 transcription)

Function: 1) essential for basal & regulated expression of nearly all RNA polymerase 2-dependent genes 2) can either repress or activate transcription 3) may act as a bridge) (conveying regulatory information from enhancers & other control elements to the promoter 4) serves as a scaffold for the assembly of a functional preinitiation complex with RNA polymerase 2 & the general transcription factors Structure: - subunits: - MED1, MED4, MED6, MED7, MED8, MED9, MED10, MED11, MED12, MED13, MED13L, MED14, MED15, MED16, MED17, MED18, MED19, MED20, MED21, MED22, MED23, MED24, MED25, MED26, MED27, MED29, MED30, MED31, CCNC, CDK8 & CDC2L6/CDK11 - the MED12, MED13, CCNC & CDK8 subunits form a distinct module termed the CDK8 module - mediator containing the CDK8 module is less active than mediator without in supporting transcriptional activation - individual preparations of the mediator complex lacking one or more distinct subunits have been variously termed ARC, CRSP, DRIP, PC2, SMCC & TRAP Notes: - it appears that several mediator complexes exist

Specific

IXL mediator complex (mediator of RNA polymerase 2 transcription) MED8 mediator complex (mediator of RNA polymerase 2 transcription) SMCC mediator complex (mediator of RNA polymerase 2 transcription)

Properties

SUBUNITS: MED1 mediator of RNA polymerase II transcription subunit 4 COMPARTMENT: cell nucleus MOTIF: coiled coil {24-48} MOTIF: Ser phosphorylation site {S32} coiled coil {90-131} serine-rich region {262-269} MOTIF: serine residue (SEVERAL) mediator of RNA polymerase II transcription subunit 6 COMPARTMENT: cell nucleus mediator of RNA polymerase II transcription subunit 7 COMPARTMENT: cell nucleus MOTIF: Ser phosphorylation site {S194} mediator of RNA polymerase II transcription subunit 8 COMPARTMENT: cell nucleus MOTIF: coiled coil {1-28} Ser phosphorylation site {S82} coiled coil {133-163} MOTIF: Elongin BC {142-151} mediator of RNA polymerase 2 transcription subunit 9 COMPARTMENT: cell nucleus MOTIF: proline-rich region SITE: 17-54 MOTIF: proline residue (SEVERAL) coiled coil {112-139} mediator of RNA polymerase 2 transcription subunit 10 COMPARTMENT: cell nucleus mediator of RNA polymerase 2 transcription subunit 11 COMPARTMENT: cell nucleus mediator of RNA polymerase II transcription subunit 12 COMPARTMENT: cell nucleus MOTIF: Tyr phosphorylation site {Y166} Ser phosphorylation site {S635} Ser phosphorylation site {S698} Ser phosphorylation site {S700} Ser phosphorylation site {S1258} Ser phosphorylation site {S1269} CTNNB1/GLI3 interaction {1616-2051} MOTIF: proline-rich region SITE: 1732-1777 MOTIF: proline residue (SEVERAL) glutamine-rich region {1900-2168} MOTIF: glutamine residue (SEVERAL) mediator of RNA polymerase II transcription subunit 13 COMPARTMENT: cell nucleus MOTIF: Thr phosphorylation site {T326} Thr phosphorylation site {T329} Thr phosphorylation site {T336} Tyr phosphorylation site {Y394} Ser phosphorylation site {S395} Ser phosphorylation site {S481} Ser phosphorylation site {S500} Ser phosphorylation site {S504} Thr phosphorylation site {T519} Ser phosphorylation site {S530} Ser phosphorylation site {S537} Thr phosphorylation site {T863} Ser phosphorylation site {S890} Ser phosphorylation site {S1029} LXXLL motif 1 {1188-1192} LXXLL motif 2 {1279-1283} serine-rich region {1508-1573} MOTIF: serine residue (SEVERAL) Ser phosphorylation site {S2048} thyroid hormone receptor-associated protein 2 COMPARTMENT: cell nucleus MOTIF: leucine zipper SITE: 1380-1401 FOR-BINDING-VIA: leucine zipper serine-rich region {1566-1610} MOTIF: serine residue (SEVERAL) mediator of RNA polymerase II transcription subunit 14 COMPARTMENT: cell nucleus MOTIF: LXXLL motif 1 {69-73} STAT2 interaction {188-566} SREBF1 interaction {500-824} MOTIF: Ser phosphorylation site {S617} Ser phosphorylation site {S986} proline-rich region SITE: 1002-1165 MOTIF: proline residue (SEVERAL) Ser phosphorylation site {S1112} Ser phosphorylation site {S1119} Ser phosphorylation site {S1128} Ser phosphorylation site {S1133} Ser phosphorylation site {S1136} Ser phosphorylation site {S1142} Ser phosphorylation site {S1144} LXXLL motif 2 {1182-1186} mediator of RNA polymerase II transcription subunit 15 COMPARTMENT: cytoplasm cell nucleus MOTIF: acetylation site SITE: N-TERMINUS EFFECTOR-BOUND: acetyl SREBF1 interaction {9-73} glutamine-rich region {161-174} MOTIF: glutamine residue (SEVERAL) glutamine-rich region {178-193} MOTIF: glutamine residue (SEVERAL) glutamine-rich region {205-218} MOTIF: glutamine residue (SEVERAL) glutamine-rich region {226-239} MOTIF: glutamine residue (SEVERAL) glutamine-rich region {243-262} MOTIF: glutamine residue (SEVERAL) proline-rich region SITE: 266-515 MOTIF: proline residue (SEVERAL) glutamine-rich region {360-367} MOTIF: glutamine residue (SEVERAL) proline-rich region SITE: 449-456 MOTIF: proline residue (SEVERAL) nuclear translocation signal {547-564} proline-rich region SITE: 602-611 MOTIF: proline residue (SEVERAL) Thr phosphorylation site {T603} mediator of RNA polymerase II transcription subunit 16 COMPARTMENT: cell nucleus MOTIF: WD repeat {79-118} WD repeat {210-252} WD repeat {275-319} WD repeat {634-675} WD repeat {790-829} mediator of RNA polymerase II transcription subunit 17 COMPARTMENT: cell nucleus MOTIF: Ser phosphorylation site {S37} Ser phosphorylation site {S50} Ser phosphorylation site {S53} Ser phosphorylation site {S55} Ser phosphorylation site {S75} Ser phosphorylation site {S76} Thr phosphorylation site {T143} Ser phosphorylation site {S185} mediator of RNA polymerase 2 transcription subunit 18 COMPARTMENT: cell nucleus MOTIF: Ser phosphorylation site {S66} mediator of RNA polymerase 2 transcription subunit 19 COMPARTMENT: cell nucleus MOTIF: proline-rich region SITE: 14-53 MOTIF: proline residue (SEVERAL) lysine-rich region {172-222} MOTIF: lysine residue (SEVERAL) mediator of RNA polymerase 2 transcription subunit 20 COMPARTMENT: cell nucleus RNA polymerase 2 holoenzyme component SRB7 COMPARTMENT: cell nucleus surfeit locus protein 5 COMPARTMENT: cell nucleus MOTIF: coiled coil {93-122} serine-rich region {136-140} MOTIF: serine residue (SEVERAL) mediator of RNA polymerase II transcription subunit 23 COMPARTMENT: cell nucleus MOTIF: Ser phosphorylation site {S1352} mediator of RNA polymerase II transcription subunit 24 COMPARTMENT: cell nucleus MOTIF: LXXLL motif 1 {128-132} LXXLL motif 2 {344-348} LXXLL motif 3 {448-452} LXXLL motif 4 {557-561} LXXLL motif 5 {788-792} LXXLL motif 6 {857-861} Ser phosphorylation site {S862} Ser phosphorylation site {S873} mediator of RNA polymerase 2 transcription subunit 25 COMPARTMENT: cell nucleus MOTIF: mediator complex interaction {1-226} proline-rich region SITE: 200-381 MOTIF: proline residue (SEVERAL) VP16 interaction {389-543} CREBBP interaction {395-545} RARA interaction {564-653} proline-rich region SITE: 565-735 MOTIF: proline residue (SEVERAL) RARA interaction {640-707} LXXLL {646-650} mediator of RNA polymerase II transcription subunit 26 COMPARTMENT: cell nucleus MOTIF: TFIIS N-terminal {10-87} proline-rich region SITE: 228-325 MOTIF: proline residue (SEVERAL) Ser phosphorylation site {S314} Ser phosphorylation site {S337} Ser phosphorylation site {S361} Ser phosphorylation site {S447} CRSP complex subunit 8 COMPARTMENT: cell nucleus mediator of RNA polymerase 2 transcription subunit 29 COMPARTMENT: cell nucleus MOTIF: glutamine-rich region {29-84} MOTIF: glutamine residue (SEVERAL) thyroid hormone receptor-associated protein 6 COMPARTMENT: cell nucleus MOTIF: coiled coil {70-94} coiled coil {133-173} mediator of RNA polymerase 2 transcription subunit 31 COMPARTMENT: cell nucleus MOTIF: glutamine-rich region {122-125} MOTIF: glutamine residue (SEVERAL) cyclin C COMPARTMENT: cell nucleus CELL-CYCLE: G1/S MOTIF: ubiquitination site (destruction box, D-box) SITE: N-TERMINAL FOR-BINDING-OF: ubiquitin PEST region cyclin box FOR-BINDING-OF: cyclin-dependent kinase cyclin-dependent kinase-8 STATE: active state MOTIF: kinase domain SITE: 21-335 MOTIF: ATP-binding site NAME: ATP-binding site SITE: 27-35 ATP-binding site NAME: ATP-binding site SITE: 52-52 aspartate residue {D151} glutamine-rich region {373-377} MOTIF: glutamine residue (SEVERAL) DC2L6

References

UniProt :accession Q9H204

Components

CRSP complex subunit 8; cofactor required for Sp1 transcriptional activation subunit 8; transcriptional coactivator; p37 TRAP/SMCC/PC2 subunit (CRSP8, CRSP34, MED27) cyclin C (CCNC, hSRB11) cyclin-dependent kinase-8 (CDK8, protein kinase K35, mediator complex subunit CDK8) mediator of RNA polymerase 2 transcription subunit 10 (mediator complex subunit 10, transformation-related gene 17 protein, TRG-17, transformation-related gene 20 protein, TRG-20, MED10) mediator of RNA polymerase 2 transcription subunit 11 (mediator complex subunit 11, MED11) mediator of RNA polymerase 2 transcription subunit 18 (mediator complex subunit 18, p28b, MED18) mediator of RNA polymerase 2 transcription subunit 19 (mediator complex subunit 19, lung cancer metastasis-related protein 1, MED19 LCMR1) mediator of RNA polymerase 2 transcription subunit 20; mediator complex subunit 20; TRF-proximal protein homolog; hTRFP (MED20, TRFP) mediator of RNA polymerase 2 transcription subunit 25 (mediator complex subunit 25, activator-recruited cofactor 92 kD component, ARC92, activator interaction domain 1 protein, p78, MED25, ACID,1 ARC92, PTOV2, TCBAP0758) mediator of RNA polymerase 2 transcription subunit 29 (mediator complex subunit 29, intersex-like protein, IXL, MED29) mediator of RNA polymerase 2 transcription subunit 31 (mediator complex subunit SOH1, hSOH1, MED31, SOH1, CGI-125) mediator of RNA polymerase 2 transcription subunit 9 (mediator complex subunit 9, MED9 MED25) mediator of RNA polymerase II transcription subunit 12; activator-recruited cofactor 240 kD component; ARC240; CAG repeat protein 45; mediator complex subunit 12; OPA-containing protein; thyroid hormone receptor-associated protein complex 230 kD component; trap230; trinucleotide repeat-containing gene 11 protein (MED12, ARC240, CAGH45, HOPA, KIAA0192, TNRC11, TRAP230) mediator of RNA polymerase II transcription subunit 13; activator-recruited cofactor 250 kD component; ARC250; mediator complex subunit 13; thyroid hormone receptor-associated protein 1; thyroid hormone receptor-associated protein complex 240 kD component; trap240; vitamin D3 receptor-interacting protein complex component DRIP250; DRIP250 (MED13, ARC250, KIAA0593, THRAP1, TRAP240) mediator of RNA polymerase II transcription subunit 14; activator-recruited cofactor 150 kD component; ARC150; cofactor required for Sp1 transcriptional activation subunit 2; CRSP complex subunit 2; mediator complex subunit 14; RGR1 homolog; hRGR1; thyroid hormone receptor-associated protein complex 170 kD component; trap170; transcriptional coactivator CRSP150; vitamin D3 receptor-interacting protein complex 150 kD component; DRIP150 (MED14, ARC150, CRSP2, CXorf4, DRIP150, EXLM1, RGR1, TRAP170) mediator of RNA polymerase II transcription subunit 15; activator-recruited cofactor 105 kD component; ARC105; CTG repeat protein 7a; mediator complex subunit 15; positive cofactor 2 glutamine/Q-rich-associated protein; PC2 glutamine/Q-rich-associated protein; TPA-inducible gene 1 protein; TIG-1; trinucleotide repeat-containing gene 7 protein (MED15, ARC105, CTG7A, PCQAP, TIG1, TNRC7) mediator of RNA polymerase II transcription subunit 16; mediator complex subunit 16; thyroid hormone receptor-associated protein 5; thyroid hormone receptor-associated protein complex 95 kD component; trap95; vitamin D3 receptor-interacting protein complex 92 kD component; DRIP92 (MED16, DRIP92, THRAP5) mediator of RNA polymerase II transcription subunit 17; activator-recruited cofactor 77 kD component; ARC77; cofactor required for Sp1 transcriptional activation subunit 6; CRSP complex subunit 6; mediator complex subunit 17; thyroid hormone receptor-associated protein complex 80 kD component; trap80; transcriptional coactivator CRSP77; vitamin D3 receptor-interacting protein complex 80 kD component; DRIP80 (MED17, ARC77, CRSP6, DRIP77, DRIP80, TRAP80) mediator of RNA polymerase II transcription subunit 23; activator-recruited cofactor 130 kD component; ARC130; cofactor required for Sp1 transcriptional activation subunit 3; CRSP complex subunit 3; mediator complex subunit 23; protein sur-2 homolog; hSur-2; transcriptional coactivator CRSP130; vitamin D3 receptor-interacting protein complex 130 kD component; DRIP130 (MED23, ARC130, CRSP3, DRIP130, KIAA1216, SUR2) mediator of RNA polymerase II transcription subunit 24; activator-recruited cofactor 100 kD component; ARC100; cofactor required for Sp1 transcriptional activation subunit 4; CRSP complex subunit 4; mediator complex subunit 24; thyroid hormone receptor-associated protein 4; thyroid hormone receptor-associated protein complex 100 kD component; trap100; hTRAP100; vitamin D3 receptor-interacting protein complex 100 kD component; DRIP100 (MED24, ARC100, CRSP4, DRIP100, KIAA0130, THRAP4, TRAP100) mediator of RNA polymerase II transcription subunit 26; activator-recruited cofactor 70 kD component; ARC70; cofactor required for Sp1 transcriptional activation subunit 7; CRSP complex subunit 7; mediator complex subunit 26; transcriptional coactivator CRSP70 (MED26, ARC70, CRSP7) mediator of RNA polymerase II transcription subunit 4; activator-recruited cofactor 36 kD component; ARC36; mediator complex subunit 4; TRAP/SMCC/PC2 subunit p36 subunit; vitamin D3 receptor-interacting protein complex 36 kD component; DRIP36 (MED4, ARC36, DRIP36, VDRIP, HSPC126) mediator of RNA polymerase II transcription subunit 6; activator-recruited cofactor 33 kD component; ARC33; mediator complex subunit 6; hmed6; renal carcinoma antigen NY-REN-28 (MED6, ARC33) mediator of RNA polymerase II transcription subunit 7; hMED7; activator-recruited cofactor 34 kD component; ARC34; cofactor required for Sp1 transcriptional activation subunit 9; CRSP complex subunit 9; mediator complex subunit 7; RNA polymerase transcriptional regulation mediator subunit 7 homolog; transcriptional coactivator CRSP33 (MED7 ARC34 CRSP9) mediator of RNA polymerase II transcription subunit 8; activator-recruited cofactor 32 kD component; ARC32; mediator complex subunit 8 (MED8) RNA polymerase 2 holoenzyme component SRB7; mediator complex subunit 21; suppressor of RNA polymerase B7 (SRB7, MED21, SURB7) surfeit locus protein 5; mediator of RNA polymerase II transcription subunit 22; mediator complex subunit 22; Surf-5 (MED22, SURF5) thyroid hormone receptor-associated protein 2 (thyroid hormone receptor-associated protein complex 240 kD component-like, THRAP2, KIAA1025, PROSIT240, TRAP240L) thyroid hormone receptor-associated protein 6 (thyroid hormone receptor-associated protein complex 25 kD component, trap25, TRAP/mediator complex component TRAP25, MED30, THRAP6, TRAP25)