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matrix metalloproteinase-20; matrixin 20; enamel metalloproteinase; enamelysin (MMP20)
Function:
- degrades amelogenin, aggrecan & COMP (cartilage oligomeric matrix protein)
- may play a role in tooth enamel formation
- autoactivates at least at the 107-Asn-|-Tyr-108 site
- cleaves aggrecan at the 360-Asn-|-Phe-361 site
Cofactor:
- binds 2 Zn+2 per subunit (putative)
- Ca+2 (putative)
Structure:
- the conserved Cys present in the cysteine-switch motif binds the catalytic Zn+2, thus inhibiting the enzyme
- dissociation of Cys from Zn+2 upon activation- peptide release activates the enzyme
- belongs to the peptidase M10A family
- contains 4 hemopexin-like domains
Compartment:
- secreted, extracellular space, extracellular matrix (putative)
Expression: expressed specifically in the enamel organ
Pathology:
- defects in MMP20 are a cause of amelogenesis imperfecta pigmented hypomaturation type
General
matrixin or matrix metalloproteinase
Properties
SIZE: entity length = 483 aa
MW = 54 kD
COMPARTMENT: extracellular matrix
MOTIF: signal sequence {1-22}
Cysteine switch {98-105}
MOTIF: Zn+2-binding site
SITE: 100-100
Zn+2-binding site
SITE: 226-226
glutamate residue {E227}
Zn+2-binding site
SITE: 230-230
Zn+2-binding site
SITE: 236-236
cysteine residue {C296}
MODIFICATION: cysteine residue {C483}
Hemopexin-like 1 {302-345}
Hemopexin-like 2 {347-389}
Hemopexin-like 3 {394-441}
Hemopexin-like 4 {443-483}
MOTIF: cysteine residue {C483}
MODIFICATION: cysteine residue {C296}
Database Correlations
OMIM correlations
UniProt O60882
PFAM correlations
Entrez Gene 9313
Kegg hsa:9313
References
- UniProt :accession O60882
- NIEHS-SNPs
http://egp.gs.washington.edu/data/mmp20/